한국응용곤충학회 학술대회논문집 2017 한국응용곤충학회 추계학술발표회 및 국제심포지엄 , 해충관리의 정책적 강화 (p.17-17)

hpRNA-induced gene silencing in Beauveria bassiana JEF-007 for gene characterization

키워드 :
Autophagy protein 22,Beaveria bassiana,Complex I intermediated-associated protein 30,hpRNAi,TAIL-PCR

초록

A genus of entomopathogenic Beaveria bassiana has been widely used in pest management, however little studies havebeen given to its virulence-related genes. To identify the roles of virulence genes, AtMT-induced random mutants weregenerated and followed by localization study with TAIL-PCR. Two genes, Complex I intermediated-associated protein30 (CIP30) and Autophagy protein 22 (Atg22), were predicted as virulence-related genes in B.bassiana JEF-007. To validatethe a possible relationship between two genes and fungal virulence, hpRNAi was performed. A hpRNAi plasmid wasconstructed as a model system to knock down of egfp gene in egfp-expressing B. bassiana transformant. A Real-TimePCR showed the knock down of egfp gene expression via hpRNAi. The CIP30 and Atg22 genes in B. bassiana willbe validated by the established RNAi technique. This work can be a strong platform for the functional genetics in B.bassiana.