한국응용곤충학회 학술대회논문집 2017 한국응용곤충학회 추계학술발표회 및 국제심포지엄 , 해충관리의 정책적 강화 (p.18-18)

Production of porcine epidemic diarrhea virus (PEDV) VLP using the baculovirus expression vector system in Bombyx mori

키워드 :
Porcine epidemic diarrhea virus (PEDV),Chimeric Virus-like particle(cVLP),BEVS,Bombyx mori

초록

Viral particles of Porcine epidemic diarrhea virus (PEDV) consist of a four structural proteins. Among them Spikeprotein mediated responsible for receptor binding and membrane fusion during viral infection and therefore the main targetof neutralizing antibodies. Virus-like particles (VLPs) are consisted of one or more viral structural proteins, and theirmorphologies closely resemble those of the native virus. VLPs have no virulence and can elicit robust immune responsesas compared with inactivated or live-attenuated virus vaccines. Thus, in this study, we tried two methods for VLP constructionin Bombyx mori, one is traditional method and the other is chimeric VLP method using the influenza matrix protein.Both methods could produce successfully PEDV VLPs.