Viral particles of Porcine epidemic diarrhea virus (PEDV) consist of a four structural proteins. Among them Spikeprotein mediated responsible for receptor binding and membrane fusion during viral infection and therefore the main targetof neutralizing antibodies. Virus-like particles (VLPs) are consisted of one or more viral structural proteins, and theirmorphologies closely resemble those of the native virus. VLPs have no virulence and can elicit robust immune responsesas compared with inactivated or live-attenuated virus vaccines. Thus, in this study, we tried two methods for VLP constructionin Bombyx mori, one is traditional method and the other is chimeric VLP method using the influenza matrix protein.Both methods could produce successfully PEDV VLPs.

• Won Seok Gwak(Department of Agricultural Biology, College of Agriculture, Life & Environment Science, Chungbuk National University)
• Ji Hoon Lee(Department of Agricultural Biology, College of Agriculture, Life & Environment Science, Chungbuk National University)
• Dong Jun Kim(Department of Agricultural Biology, College of Agriculture, Life & Environment Science, Chungbuk National University)
• Hwi Geon Yun(Department of Agricultural Biology, College of Agriculture, Life & Environment Science, Chungbuk National University)
• Ji In Ma(Department of Agricultural Biology, College of Agriculture, Life & Environment Science, Chungbuk National University)
• Soo Dong Woo(Department of Agricultural Biology, College of Agriculture, Life & Environment Science, Chungbuk National University)