In this study, we investigated the effect of bisphosphonate on the osteoblastic differentiation of human dental stem cells (hDPSCs). In the first experiment, we evaluated the effect of bisphosphonate on the differentiation of hDPSCs into osteoblasts by alkaline phosphatase staining after culturing hDPSCs. As a result, on day 13, the osteogenic differentiation of hDPSC was suppressed at 5 μM in clodronate and 2 μM in zolendronate. In NBP, osteogenic differentiation is more suppressed. In second experiment, cytotoxicity and proliferation test, the cell proliferation (examined by MTT assay) was more suppressed as the concentrations of zolendronate were larger than those of alendronate and clodronate. Western blotting, a third experiment, was found that AKT phosphorylation was inhibited in cell signaling proteins involved in cell proliferation inhibition and death by bisphosphonate concentration. In human dental stem cells, bisphosphonates inhibit osteoblast differentiation, and this phenomenon is clearly observed in NBPs (zolendronate), and it has been found that it is related to AKT phosphorylation of cell signaling proteins.

• 오종식(부산대학교 치의학전문대학원 구강악안면외과학교실) | Jong-Shik Oh (Department of Oral and Maxillofacial Surgery)
• 유미현(구강병리학교실) | Mi-Heon Ryu (Department of Oral Pathology)
• 김형준(구강생리학교실) | Hyung-Joon Kim (Department of Oral Physiology, School of Dentistry, Pusan National University)
• 신상훈(부산대학교 치의학전문대학원 구강악안면외과학교실) | Sang-Hun Shin (Department of Oral and Maxillofacial Surgery)
• 이재열(부산대학교 치의학전문대학원 구강악안면외과학교실) | Jae-Yeol Lee (Department of Oral and Maxillofacial Surgery) Corresponding author