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어피 펩타이드 리포좀 대량생산 최적 조건 및 저장 안정성 KCI 등재

Optimization and Scale-up of Fish Skin Peptide Loaded Liposome Preparation and Its Storage Stability

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산업식품공학 (Food Engineering Progress)
한국산업식품공학회 (Korean Society for Food Engineering)
초록

Fish skin peptide-loaded liposomes were prepared in 100 mL and 1 L solution as lab scales, and 10 L solution as a prototype scale. The particle size and zeta potential were measured to determine the optimal conditions for the production of fish skin peptide-loaded liposome. The liposome was manufactured by the following conditions: (1) primary homogenization at 4,000 rpm, 8,000 rpm, and 12,000 rpm for 3 minutes; (2) secondary homogenization at 40 watt (W), 60 W, and 80 W for 3 minutes. From this experimental design, the optimal conditions of homogenization were selected as 4,000 rpm and 60 W. For the next step, fish peptides were prepared as the concentrations of 3, 6, and 12% at the optimum manufacturing conditions of liposome and stored at 4ºC. Particle size, polydispersion index (pdI), and zeta potential of peptide-loaded liposome were measured for its stability. Particle size increased significantly as manufacture scale and peptide concentration increased, and decreased over storage time. The zeta potential results increased as storage time increased at 10 L scale. In addition, 12% peptide showed the formation of a sediment layer after 3 weeks, and 6% peptide was considered to be the most suitable for industrial application.

목차
서 론
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 References
저자
  • 이중규(건국대학교 축산식품생명공학과, Department of Food Science and Biotechnology of Animal Resources, Konkuk University) | JungGyu Lee
  • 이윤정(건국대학교 축산식품생명공학과, Department of Food Science and Biotechnology of Animal Resources, Konkuk University) | YunJung Lee
  • 백정정(건국대학교 축산식품생명공학과, Department of Food Science and Biotechnology of Animal Resources, Konkuk University) | JingJing Bai
  • 김수진((주)다인소재 소재과학연구소, Materials Science laboratory R&D Center, Dyne soze additives and ingredients) | Soojin Kim
  • 조영재(건국대학교 축산식품생명공학과,건국대학교 동물자원센터, Department of Food Science and Biotechnology of Animal Resources, Konkuk University, Animal Resources Research Center, Konkuk University) | Youngjae Cho
  • 최미정(건국대학교 축산식품생명공학과, Department of Food Science and Biotechnology of Animal Resources, Konkuk University) | Mi-Jung Choi Corresponding author