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금잔화, 상황, 어성초 에탄올 추출물의 세포독성 평가 및 3T3-L1 세포에 대한 육모 효과 KCI 등재
Cell Viability and Hair Growth Effect on 3T3-L1 Cells of Ethanol Extract from Calendula officinalis L. Flower, Phellinus linteus Fruit Body and Houttuynia cordata Thunb. Whole Plant
pp.404-410
Background: Hair loss related syndromes are increasing due to environmental pollution and stress. Hair care products are mainly prepared by mixing chemicals and natural extracts, such as those obtained from medicinal plants. The purpose of this study was to investigate the effects of 70% ethanol extracts from the flowers of Calendula officinalis, fruit body of Phellinus linteus, and the whole plant of Houttuynia cordata on the growth of CCD-986 cells, hair follicle dermal papilla cells (HFDPC), and 3T3-L1 cells. Methods and Results: All sample extracts at all concentrations, except for that from P. linteus fruit body at 500㎍/㎖, were cytotoxic to CCD-986 cells. However, none of the sample extracts were cytotoxic to HFDPC. The lipid differentiation of 3T3-L1 cells regulates hair regeneration via secretion of platelet derived growth factor. The 70% ethanol extract of H. cordata whole plant promoted hair growth. Adipogenesis rate significantly increased in a treatment concentration-dependent manner. Conclusions: These results suggest that 70% ethanol extracts of C. officinalis flower, P. linteus fruit body and H. cordata could be used for the development of hair care products.
서 언
재료 및 방법
1. 실험 재료
2. 총 폴리페놀 함량 측정
3. 총 플라보노이드 함량 측정
4. 세포 배양
5. 세포독성 측정
6. 지방전구세포 분화 확인
7. Oil red O staining
8. 통계 처리
결과 및 고찰
1. 총 폴리페놀 및 총 플라보노이드 함량
2. 섬유아세포에 대한 독성 평가
3. 모유두세포에 대한 독성 평가
4. 지방전구세포 독성
5. 지방 분화 확인
감사의 글
REFERENCES
