논문 상세보기
PCR (polymerase chain reaction)법과 조직학적 방법을 통한 넙치 조직에서의 Kudoa septempunctata (Myxosporea: Multivalvulida)의 분석에 관한 연구 KCI 등재
A Study About Analysis Results for Kudoa septempunctata (Myxosporea: Multivalvulida) in Tissue at Olive Flounder, using PCR (polymerase chain reaction) and Histological Methods
This study is for the consideration of the existence tendency of Kudoa septempunctata in olive flounder. In general, muscle has shown a strong PCR positive reaction in spores containing tissues rather than non-containing tissues. However, blood PCR results showed opposed tendency. In various organs of the tested fish containing spores in muscle tissue, heart had shown positive reaction along with muscle at PCR analysis. Muscle fiber necrosis was observed at the histological observation, and this degeneration was common in both samples. The one sample was the PCR positive muscle containing spore and the other was the PCR positive muscle non-containing spore. Both of muscle tissues indicated a positive reaction at ISH (in-situ hybridization) against K. septempunctata.
재료 및 방 법
1. 실험어
2. 양성체 선별
3. 순치 2개월 후 샘플링
3. 순치 2개월 후 샘플링
4. 조직학적 관찰
5. 분자조직학적 관찰 (In-situ hybridization: ISH법)
결과 및 고 찰
1. 근육과 혈액의 KS-PCR 검사 결과
2. 각 장기에서의 KS-PCR 검사 결과
3. 조직학적, 분자조직학적 관찰 결과
적 요
REFERENCES
