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어성초(Houttuynia cordata Thunb) 유산균 발효물의 항알러지 활성 KCI 등재

Anti-allergic Activity of the Extracts from Houttuynia cordata Thunb Fermented by Lactic Acid Bacteria

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한국환경과학회지 (Journal of Environmental Science International)
한국환경과학회 (The Korean Environmental Sciences Society)
초록

This study was performed to evaluate the possibility of application of lactic acid bacteria fermentation to increase the anti-allergic activity of the extracts from Houttuynia cordata Thunb. H. cordata Thunb was fermented on 25, 30, 35 and 40℃ for 5 days by two species of lactic acid bacteria, Leuconostoc mesenteroides 4395 and Lactobacillus sakei 383. The anti-allergic activity of water extracts of H. cordata Thunb was then analyzed both before and after fermentation. Anti-allergic activity was determined in vitro assays by using 5-lipoxygenase (5-LO), cyclooxygenase-2 (COX-2) and β-hexoseaminidase release of RBL-2H3 cells (degranulation marker). The extracts fermented at 35℃ by both bacteria had the highest inhibitory activities against 5-LO, and also higher than the control, and the one fermented at 30℃ by both bacteria had the highest inhibitory activity against COX-2. The degranulation of RBL-2H3 cells induced by IgE-antigen complex was estimated as β-hexoseaminidase release rate as reference of 100%, the release rates were inhibited in 25 μg/ml of the extracts fermented at 30, 35 and 40℃ only by L. mesenteroides 4395. These results indicate that anti-allergic activity of H. cordata Thunb is increased by lactic acid bacteria fermentation.

목차
Abstract
 1. 서 론
 2. 재료 및 방법
  2.1. 재료
  2.2. 시료의 추출 및 발효
  2.3. 5-LO 억제 효능 측정
  2.4. Cyclooxygenase-2 (COX-2) 억제 효능 측정
  2.5. 탈과립 억제효능 측정
  2.6. 통계처리
 3. 결과 및 고찰
  3.1. 5-LO 억제 효능
  3.2. COX-2 억제 효능
  3.3. 탈과립 억제효능
 4. 결 론
 감사의 글
 REFERENCES
저자
  • 이영근(부산대학교 식품공학과) | Young-Guen Lee
  • 김용민(부산대학교 식품공학과) | Yong-Min Kim
  • 정해진(부산대학교 식품공학과) | Hae-Jin Jeong
  • 이보경(부산대학교 약학대학 MIRCA) | Lee Bo kyung
  • 임동순(부산대학교 약학대학 MIRCA) | Dong-Soon Im
  • 이승은(CJ제일제당 소재연구소) | Sung-Eun Lee
  • 김한수(부산대학교 식품공학과) | Han-Soo Kim Corresponding author