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운목향 70% 에탄올 추출물의 항염증 및 면역조절에 대한 효과 KCI 등재

Anti-inflammatory and Immune Regulatory Effects of Aucklandia lappa Decne 70% Ethanol Extract

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한국약용작물학회지 (Korean Journal of Medical Crop Science)
한국약용작물학회 (The Korean Society of Medicinal Crop Science)
초록

Background: This present study was conducted to evaluate the anti-inflammatory and immune regulatory effects of Aucklandia lappa Decne (AL). Methods and Results: We measured cytotoxicity, nitric oxide (NO) content, mRNA expression (iNOS, IL-1α, IL-1β, and TNF-α), protein expression (iNOS, COX-2, and IκB-α) and phagocytic activity in RAW264.7 cells. Male BALB/c mice were fed 100 ㎎/㎏ AL (Aucklandia lappa Decneon 70% ethanol extract) and 250 ㎎/㎏ AL for 4 weeks; thereafter, we observed B/T or CD4+/CD8+ lymphocyte subpopulation change, and expression patterns of CD4+ and CD8+ lymphocytes by immunohistochemical staining in mouse splenocytes and/or thymocytes. To determine the experimental concentration of AL, cell viability was measured by MTT assay and tested at 12.5 ㎍/㎖ or less. AL inhibited the levels of NO, lymphokine production (IL-1β, and TNF-α), and mRNA (iNOS, IL-1α, IL-1β, and TNF-α) and protein (iNOS, and COX-2) expression. Additionally, the levels of IκB-α, phagocytic activity, and splenic and thymic T lymphocytes, especially TH and TC cells were significantly increased in AL administered mice. The immuno-reactive density of CD4+ and CD8+ lymphocytes was stronger in AL groups than in the normal group. AL stimulated NO, iNOS, and COX-2, and regulated IL-1α, IL-1β, TNF-α, and IκB-α in macrophages treated with LPS (lipopolysaccharide). In addition, AL increased the phagocytic activity of macrophages and the immunity of mouse T (TH, and TC) cells. Conclusions: These results suggested that AL might show anti-inflammatory activity via the suppression of various inflammatory markers and immuno-regulatory activity.

목차
서 언
 재료 및 방법
  1. 실험 재료
  2. 세포주 및 실험동물
  3. 세포생존율 (MTT assay)
  4. 대식세포의 NO 측정
  5. RT (reverse transcription) PCR
  6. Cytokines (IL-1β, TNF-α) 측정
  7. 단백질 분리 및 웨스턴 블로팅
  8. 탐식작용 활성 측정
  9. 비장 및 흉선림프구 아집단 측정
  10. 면역조직화학적 염색
  11. 통계처리
 결 과
  1. 대식세포 (RAW264.7 세포주) 생존율에 미치는 효과
  2. NO 생성 억제에 미치는 효과
  3. 염증 관련 cytokines (iNOS, IL-1α, -1β 및 TNF-α)mRNA 발현 효과
  4. 염증관련 cytokines (IL-1β, TNF-α) 분비 억제 효과
  5. 염증관련 단백질 (iNOS, COX-2, IκB-α) 발현 효과
  6. 대식세포주의 탐식활성에 미치는 효과
  7. 면역세포 아집단에 미치는 효과
  8. CD4+/CD8+ T 림프구의 면역조직화학적 변화
 고 찰
 REFERENCES
저자
  • 김민선(우석대학교 식품생명공학과, Department of Food Biotechnology, Woosuk University) | Min Sun Kim
  • 김남석(우석대학교 식품생명공학과, Department of Food Biotechnology, Woosuk University) | Nam Seok Kim
  • 권진(한국복지대학교 의료보장구과, Department of Prosthetics and Orthotics, Korea National University of Welfare) | Jin Kwon
  • 김하림(우석대학교 식품생명공학과, Department of Food Biotechnology, Woosuk University) | Ha Rim Kim
  • 이다영(우석대학교 식품생명공학과, Department of Food Biotechnology, Woosuk University) | Da Young Lee
  • 오미진(한국식품연구원, Korea Food Research Institute, Wanju 55365, Korea) | Mi Jin Oh
  • 김홍준(우석대학교 한의예과, Department of Korean Medicine, Woosuk University) | Hong Jun Kim
  • 이창현(우석대학교 한의예과, Department of Korean Medicine, Woosuk University) | Chang Hyun Lee
  • 오찬호(우석대학교 식품생명공학과, Department of Food Biotechnology, Woosuk University) | Chan Ho Oh Corresponding author