Background: Polysaccharides are the most important functional constituent in Astragalus membranaceus. The purpose of the present study was to evaluate the effect of polysaccharides isolated from the aboveground parts of A. membranaceus (AMA) and polysaccharides isolated from the roots of A. membranaceus (AMR) immune function by modulated cytotoxic T cell and Th1- and Th2- related cytokines kinetics. Methods and Results: Sprague-Dawley rats were randomly divided into exhaustive exercise case groups and non-exercise case, AMA and AMR samples were administered orally for 30 days (500 ㎎/㎏/day and 10 ㎎/㎏/day, respectively) and were compared to those rats in the groups fed commercial sports drink (SPD) and vehicle. Both exhaustive exercise groups and non-exercise groups had a lower ratio of CD4+ and CD8+ cells in the spleens of the rat fed AMA and AMR compared to those in the rats fed SPD and vehicle group. These results suggested that AMA and AMR promote an increase in the proportion of cytotoxic T cells. The IL-4- producing T lymphocytes decreased significantly in the AMR (10 ㎎/㎏/day) group compared to SPD and vehicle, whereas the AMA group increased the IL-4 concentration more than the SPD and vehicle in exhaustive exercise group. However, the populations of IFN-γ-producing T lymphocytes of AMR and AMA increased. AMA decreased the concentration of IFN-γ to inhibit the Th1 response and thereby increased the concentration of IL-4 to induce a Th2 response that was related to humoral immunity in the non-exercise group. Conclusions: These results showed that, in addition to Th1/Th2 regulation, AMR and AMA played an important immuno-modulatory role after exhaustive exercise-induced Th1/Th2 lymphocyte imbalance, which might be correlated with cytokine producing immunoregulatory cells.

서 언
 재료 및 방법
  1. 실험동물
  2. 황기 지상부로부터 다당체의 제조
  3. 황기 지상부 및 지하부 다당체 경구투여
  4. 실험동물의 운동검사
  5. 실험동물에서의 면역능 검정
 결과 및 고찰
  1. 실험동물 한계운동 후 CD4+, CD8+의 세포수에 대한황기 지하부 및 지상부 다당체의 영향
  2. 실험동물 한계운동 후 cytokine (IL-2, IL-4, IFN-γ) 함량및 Th1/Th2 balance에 대한 황기 지하부 및 지상부 다당체의 영향
 REFERENCES

• 이시영(강원대학교 생약자원개발학과, Department of Herbal Medicine Resource, Kangwon National University) | Si Young Lee
• 이한나(강원대학교 생약자원개발학과, Department of Herbal Medicine Resource, Kangwon National University) | Hannah Lee
• 고은지(강원대학교 생약자원개발학과, Department of Herbal Medicine Resource, Kangwon National University) | Eun Ji Go
• 박영철(대구가톨릭대학교 GLP센터, GLP Center, Catholic University of Daegu) | Yeong Chul Park
• 최선강(강원대학교 농생명산업학과, Department of Agricultural Life Science, Kangwon National University) | Seon Kang Choi
• 유창연(강원대학교 식물자원응용공학과, Department of Applied Plant Sciences, Kangwon National University) | Chang Yeon Yu
• 임정대(강원대학교 생약자원개발학과, Department of Herbal Medicine Resource, Kangwon National University) | Jung Dae Lim Corresponding author