한국자원식물학회지 Vol.30 No.6 (p.618-622)

Isolation and Identification of Tyrosinase Inhibitors from Loranthus tanakae

키워드 :
Loranthus tanakae,Mistletoe,Rhamnocitrin 3-rhamnoside,Tyrosinase inhibitor

목차

Abstract
Introduction
Materials and Methods
  Extraction and partition
  HPLC analysis of EtOAc fraction
  Purification of EtOAc extract
  Structure analysis
  Assay of mushroom tyrosinase inhibitory activity
  Statistical analysis
Results and Discussion
  Tyrosinase inhibitory activity of solvent-fractionated layers
  Isolation of tyrosinase inhibitors from the EtOAc layers
  Identification of the isolated compounds
  Tyrosinase inhibitory activity of the isolated compounds
References

초록

Various bioactive substances are found in Loranthus tanakae, including quercetin 3-rhamnoside (1), kaempferol 3-rhamnoside (2), rhamnetin 3-rhamnoside (3), and rhamnocitrin 3-rhamnoside (4), which inhibit tyrosinase. These compounds are mainly found in the EtOAc fraction of L. tanakae extract and demonstrate higher rates of tyrosinase inhibition than ascorbic acid, which was used as a control. Our results suggest that L. tanakae extracts can be utilized in skin whitening cosmetics.