한국자원식물학회지 Vol.30 No.6 (p.647-653)

Ca2+-ATPase and cAMP-mediated Anti-Apoptotic Effects of Acanthopanax senticosus Extracts on Ischemia/Reperfusion Liver Damages

키워드 :
Acanthopanax senticosus,Apoptosis,Ca2+-ATPase,cAMP,Hepatic ischemia-reperfusion injury

목차

Abstract
Introduction
Materials and Methods
  Materials
  Animal experiments
  Preparation of hepatic ischemia-reperfusion model
  Specimen collection and testing
  Measurement of biochemical parameters
  Ca2+-ATPase activities assay
  Immunohistochemistry
  cAMP measurement
  Statistical analysis
Results
  Effects of ASI injection on ALT and AST level in serum
  Effects of ASI on activities of serum SOD, MDA, andMPO in HIRI rat
  Effects of ASI on SOD activity, MDA content, andCa2+-ATPase activity in tissue of HIRI rats
  Effects of ASI on cAMP level in liver tissue of HIRI rats
  Effects of ASI on expression of Bcl-2 and Bax in tissue ofHIRI rats
Discussion
References

초록

Hepatic ischemia-reperfusion injury (HIRI) is linked with high mortality rate. Several agents have been developed so far to reduce the risk of HIRI. In this study, we investigated the effects of Acanthopanax senticosus extract (AS) on hepatic ischemia-reperfusion. To explore the protective effects of A. senticosus extract injection (ASI) on hepatic ischemia-reperfusion injury rats animal model were used. After the development of HIRI by using clamping method rats were then randomly divided into five groups. Different doses of AS were administered in HIRI rat model. The level of ALT, AST, and MDA content in serum were detected in sham and HIRI groups. The activity of SOD, MPO and Ca2+-ATPase, content of MDA, and cAMP in hepatic tissue were also measured. Expression of Bcl-2 and Bax protein were detected by immunohistochemical staining method. Compared with sham group, ASI has the protective effect on the HIRI model in rats. Blood levels of ALT, AST, SOD, MPO, and MDA were significantly lower in ASI group compared with HIRI. Indeed SOD and Ca2+-ATPase activities, MDA content, and cAMP level were improved in ASI group. Furthermore, Bcl-2 and Bax protein were improved in ASI group compared with only HIRI group. These results suggest that AS may provide potential ameliorative therapy by inhibiting the damage signaling mechanism in hepatic ischemia/reperfusion injury model.