The aim of this study was to determine the suitability of surfactant to extract higher phenolic compound, flavonoid and antioxidant activity from Tartary buckwheat and evaluate the potentiality of surfactant as a screening agent for breeding purpose. Primarily, we employed two types of surfactant (Hydrophilic: Tween 20 and Lipophilic: Span 80) to select the suitable surfactant agent for the extraction of optimum bioactive compounds. Between two surfactants, Tween 20 showed highest efficiency at 4 mM concentration to extract total phenolic content (TP), total flavonoid (TF) and antioxidant activity (AA). Tween 20 at 4 mM concentration was fixed for further analysis along with hot water (90℃) treatment as a control. In our findings, highest TP (118 ㎎/g), TF (38 ㎎/g) and AA (76%) was achieved in KW21 and KW22 among the fifteen accessions of Tartary buckwheat. In other way, TP, TF and AA was 200%, 120% and 110% higher in surfactant formulation compared with control treatment, respectively.

Abstract
 Introduction
 Materials and Methods
  Tartary buckwheat flour preparations
  Chemical and reagents
  Sample preparation of Tartary buckwheat
  Determination of total phenolic contents (TPC)
  Determination of flavonoid content (TF)
  DPPH free radical scavenging activity (AA)
  Statistical analysis
 Results and Discussion
  Effect of surfactant types and concentration on extractionof total phenols, flavonoids and antioxidant activity ofTartary buckwheat
  Extraction of total phenols, flavonoids and antioxidantactivity of different accessions of Tartary buckwheat insurfactant formulation
 References

• Cheol Ho Park(Department of Bio-Health Technology, Kangwon National University)
• Md Obyedul Kalam Azad(Department of Bio-Health Technology, Kangwon National University)
• Jeong Won Heo(Department of Bio-Health Technology, Kangwon National University)
• Wie Soo Kang(Department of Bio-Health Technology, Kangwon National University) Corresponding author