한국식품위생안전성학회지 Vol.32 No.1 (p.70-74)

해양생물 추출물의 대장암세포주에 대한 항암 작용 검색

Anti-cancer Effect of Marine Resources Against Human Colorectal Cancer Cells
키워드 :
Colorectal cancer cell,Cytotoxicity,Marine resources,Screening

목차

Materials and Methods
  실험재료
  세포 배양
  Cell Counting Kit-8 (CCK-8) assay
  Western blot analysis
  통계학적 분석
Results and Discussion
Acknowledgement
국문요약
References

초록

Recently, the area of marine resources has become concerned with sources for the next generation of the bio-industry. Until present, development of the marine resources has remained limited, although a large number of these resources are considered to have potential for various significant biological activities. Most marine sponges, marine algae and coral could be used to create specific compounds for survival against a harsh environment. Therefore, it was necessary that these materials needed to be elucidated with biological activities, such as like anti-inflammatory, anti-viral or anti-cancer effects for their utilization in the bio-industry. In this study, we screened extracts of marine resources for their anti-cancer effect on human colorectal cancer cells. These resources were collected at Kosrae of Micronesia on April, 2013 and extracted with methanol. Cytotoxicity of marine resources was observed. Of a total of 20 specimens, three specimens dose-dependently demonstration inhibition of cell viability. Furthermore, cells treated with these specimens for 48h were induced p53, p21, Bax and caspase-3. The results suggest that they involved p53-mediated apoptosis. Two positive specimens (1304KO-327 and 1304KO-329) were verified as the identical materials, which are Hyrtios sp. Unfortunately 1304KO-207 was not yet classified and needed to identify in the further study. There results suggested that marine resources with positive potential in anticancer effect would be good candidates as useful bio-resources.