한국식품영양학회지 Vol. 31 No. 2 (p.252-257)

단풍마 단백질 추출물의 스트레스로 인한 면역력 저하 개선 효과

Immunopotentiating Effect of Protein Extract from Dioscorea quinqueloba in Stressed Mice
키워드 :
Dioscorea quinqueloba,immunopotentiation,stress,protein extract,immunity

목차

Abstract
서 론
재료 및 방법
  1. DQP-1의 분리 정제
  2. DQP-1의 성분 분석
  3. 실험 동물의 저온 스트레스 유도
  4. 실험 동물에서 가슴샘 T세포 소집단의 분포 분석
  5. 실험 동물에서 아스코르브산 농도 분석과 혈청 내 싸이토카인 농도 및 효소 활성도 분석
  6. 통계적 분석법
결 과
  1. DQP-1의 조성
  2. 저온 스트레스에 의한 면역 장기들의 변화와 DQP-1의처리 효과
  3. 저온 스트레스에 의한 가슴샘 T세포 소집단 분포 및혈중 면역 싸이토카인 농도 변화와 DQP-1의 처리 효과
  4. 저온 스트레스에 의한 혈청 내 효소 활성도의 변화와DQP-1의 처리 효과
고찰 및 결론
References

초록

It is noted that Dioscorea quinqueloba is a medicinal herb that is widely used to treat cardiovascular disease and is assessed as useful to treat other various medical conditions. The immunopotentiating effects of the protein extract (DQP-1) from Dioscorea quinqueloba were thus formally investigated in vivo under incident of cold stress. In this case study, the spleen and thymus weight in mice was shown to have decreased after a measured exposure to cold stress, while the adrenal gland weight in the mice was shown to have increased. The systematic oral administration of DQP-1 significantly recovered the weight loss of the spleen and suppressed the adrenal gland hypertrophy during the association with cold stress. Additionally, the DQP-1 also restored the ascorbic acid level in the adrenal gland reduced after cold stress. The cold stress exposure lowered the percentage of CD4+ and CD8+ cells in the mouse thymus as determined by the flow cytometric analysis, as well as the levels of some serum immunological cytokines(interleukin-2, interleukin-12, and interferon-γ) in the studied mice. The resulting identified weakened immunity caused by cold stress was also recovered by a treatment with DQP-1. The DQP-1 significantly suppressed the formation of serum enzymes of alanine aminotransferase, aspartate aminotransferase, and lactate dehydrogenase, which were systematically elevated during the cold stress episode. These results indicate that DQP-1 can improve immunity in mice that are characteristically weakened under stress.