韓國食生活文化學會誌 제33권 제2호 (p.104-111)

버드나무(Salix Koreensis Andersson) 가지 추출물의 항산화 및 항염증 효과

Antioxidant Activity and Anti-inflammatory Effects of Salix Koreensis Andersson Branches Extracts
키워드 :
Salix Koreensis andersson,SOD-like activity,anti-inflammatory effects,anti-oxidative effects,raw 264.7 macrophages

목차

Abstract
I. 서 론
II. 연구내용 및 방법
  1. 버드나무 가지 추출
  2. 항산화 활성 분석
  3. 항염증 효과 분석
   1) 세포 배양
   2) 세포 독성 측정(MTT assay)
   3) Nitric oxide (NO) 생성량 측정
   4) TNF-α, IL-6 의 측정
  4. 통계처리
III. 결과 및 고찰
  1. 총 폴리페놀 및 총 플라보노이드 함량 측정
  2. 항산화 효능 측정
   1) SOD 유사 활성과 EDA 활성
  3. 항염증 효능 측정
   1) 세포 독성 측정(MTT assay)
   2) Nitric oxide(NO) 생성량 측정
   3) Cytokine 함량( TNF-α, IL-6 측정)
IV. 요약 및 결론
Conflict of Interest
References

초록

This study aims to compare and analyze a willow tree (Salix Koreensis andersson) extract’s antioxidant and antiinflammatory activity by investigating its: total polyphenol, flavonoid content, SOD-like activity, DPPH vitality. the willow tree was induced with LPS to determine its active anti-inflammatory effects. as a result, the willow methanol extract showed a higher total polyphenol and flavonoid content than those of willow distilled water extract, but the willow distilled water extract showed a higher SOD than that of willow methanol extract. in its DPPH scavenging ability, the willow methanol extract’s antioxidant activity was higher than that of the willow distilled water extract. the willow extract’s measurements such as the production of NO, inflammatory cytokine (TNF-α, IL-6 measurement) were significantly reduced as its concentration level went down. according to the research outcomes, when induced, he will extract’s macrophage produces mediator-like substances such as NO and inflammatory cytokine that can be used to alleviate the inflammatory response. therefore, the willow tree proved to be a useful raw plant material for the products designed to combat inflammatory activities due to its natural antioxidant and anti-inflammatory response substances such as NO and cytokine.