In this study, PAT protein of genetically modified maize was prepared from the recombinant E. coli strain BL21 (DE3), and mice were immunized with the recombinant PAT protein. After cell fusion and cloning, two hybridoma cells (PATmAb-7 and PATmAb-12) were chosen since the monoclonal antibodies (Mabs) produced by them were confirmed to be specific to PAT protein in the indirect enzyme-linked immunsorbent assay (ELISA) and western blot tests. There were no cross-reactions of either Mabs to other GM proteins or to the extracts of non-GM maize. The ELISA based on the PATmAb-7 can sensitively detect 0.3 ng/g PAT protein in corn. These results indicate that the developed Mabs can be used as bio-receptors in the development of immunosensors and biosensors for the rapid and simple detection of GM corn adulterated in foods.

ABSTRACT
 Materials and Methods
  실험재료 및 기기
  PAT 단백질의 확보
  단크론성 항체 확보를 위한 hybridoma 세포주 개발
  PAT에 대한 단크론성 항체 생산 및 정제
  생산된 PAT 단크론성 항체의 특성 확인
 Results and Discussion
  재조합 PAT 단백질의 생산 및 정제
  재조합 PAT 단백질의 항원성 확인 및 hybridoma 개발
  단크론성 항체 정제 및 특성 확인
 국문요약
 References

• 김솔아(경상대학교 응용생명과학부) | Sol-A Kim (Division of Applied Life Science, Graduate School, Gyeongsang National University)
• 이정은(경상대학교 응용생명과학부) | Jeong-Eun Lee (Division of Applied Life Science, Graduate School, Gyeongsang National University)
• 심원보(경상대학교 농화학식품공학과, 경상대학교 농업생명과학연구원) | Won-Bo Shim (Department of Agricultural Chemistry and Food Science & Technology, Gyeongsang National University, Institute of Agriculture and Life Science, Gyeongsang National University)
• 강성조(㈜매디랩) | Sung-Jo Kang (MADI-LAB, Inc.)
• 정덕화(경상대학교 농화학식품공학과) | Duck-Hwa Chung (Department of Agricultural Chemistry and Food Science & Technology, Gyeongsang National University) Correspondence to