한국식품위생안전성학회지 Vol.33 No.3 (p.185-192)

수산가공식품 중 알레르겐 고등어 혼입여부 신속 검출을 위한 간접효소면역분석법의 개발

Development of an Indirect Enzyme-Linked Immunosorbent assay for Rapid Detection of Adulteration of Food Allergen Mackerel in Processed Marine Foods
키워드 :
Mackerel,Indirect ELISA,Processed marine food,Authenticity,Adulterationㅍ

목차

ABSTRACT
Materials and Methods
  시료 및 재료
  단크론성 항체 생산
  간접효소면역분석법(indirect enzyme-linked immunosorbentassay; indirect ELISA)의 개발
  수산가공식품 중 고등어 검출
Results and Discussion
  단크론성 항체 선택
  Indirect ELISA법의 최적화
  개발된 indirect ELISA법의 평가
  수산 가공 식품 중 고등어 어육 검출
국문요약
References

초록

The purpose of this study was to develop an indirect enzyme-linked immunosorbent assay (indirect ELISA) based on a monoclonal antibody (MAb) that is specific to mackerel thermal stable-soluble protein (TSSP), that can be used for the rapid detection of mackerel in processed marine foods. Among the four MAbs (3A5-1, 2, 9, and 12) developed in previous studies, the 3A5-2 MAb that showed high specificity and sensitivity were selected and used to develop the indirect ELISA method. The detection range of the indirect ELISA was 0.02%-0.001% and the detection limit of 0.001% was shown. No cross-reaction to other marine products and food ingredients was observed by the indirect ELISA. Processed marine foods containing mackerel with ≥ 0.3 O.D. value at 405 nm were estimated as positive samples by the indirect ELISA. Therefore, the indirect ELISA can be used as a rapid and sensitive method to identify mackerel authenticity and adulteration in processed marine foods.