한국약용작물학술대회 발표집 2018 한국약용작물학회 춘계학술발표회 , 나고야 의정서 발효에 따른 약용산업자원의 이용 및 대응전략 심포지엄 (p.5-5)

|학술발표회|
Pediococcus pentosaceus로 발효된 황기 지하부가 인간 피부 섬유아세포의 콜라겐 생성에 미치는 효과

Effect of Pediococcus pentosaceus Fermented Radix Astragali on Collagen Production in Human Skin Fibroblasts

초록

Background: This study were to investigate the effect of Pediococcus pentosaceus fermented Radix astragali (AMRP) and non-fermented products (AMRNP) on collagen synthesis in the cultures of human dermal fibroblasts, and their inhibitory effects on the matrix-degrading enzymes (collagenase, elastase, and gelatinase).
Methods and Results: Both AMRP and AMRNP significantly improved cell growth and proliferation of HDF cells. However, the enzyme-linked immunosorbent assay and Western blot analysis demonstrated that AMRP, but not AMRNP, significantly and dose-dependently stimulated the biosynthesis of type I procollagen in both aged (74 y) and young (21 y) HDF cells. Real-time reverse transcription-polymerase chain reaction revealed that expression of type I, type III procollagen and transforming growth factor β1 (TGF-β1) mRNA was significantly stronger in AMRP-treated HDF cells than that of AMRNP-treated and un-treated HDF cells. The AMRP revealed an increase in astragaloside Ⅳ only depending on increase in fermentation period, because other astragalside converted to astragaloside Ⅳ, which it detached acyl group by fermentation processing of Pediococcus pentosaceus.
Conclusion: The results also suggested that AMRP could stimulate the collagen biosynthesis in human dermal fibroblasts, which is, associated with the regulation of procollagen biosynthesis resulting from AMRP-induced TGF-β1 expression and the mitogenic activity in HDF cells, and therefore, is expected to reduce the age-dependent loss of extracellular matrix proteins.