동물자원연구 제29권 2호 (p.42-49)

C/EBPβ 와 C/EBPδ 유전자들과 더러브렛의 운동 역할에 관한 분자생물학적 분석

Molecular Analysis of C/EBPβ and C/EBPδ Genes and Their Role After Exercise in Thoroughbred Horses
키워드 :
C/EBPβ,C/EBPδ,gene expression,muscle,Thoroughbred horse

목차

ABSTRACT
Ⅰ. INTRODUCTION
Ⅱ. MATERIALS AND METHODS
  Preparation of horse samples and ethics statement
  RNA extraction and reverse transcription
  Real-time RT-PCR amplification
  Statistical analysis
Ⅲ. RESULTS AND DISCUSSION
  Evolutionary and phylogenetic analysis of C/EBPβ andC/EBPδ
  Differential expression patterns of Equine C/EBPβ andC/EBPδ transcripts after exercise
Ⅳ. REFERENCES

초록

As a transcription factor, the CCAAT/enhancer binding protein (C/EBP) induces the expression of specific genes by interacting with their promoters. C/EBP can trigger to express other genes, because it can open chromatin structure after binding to DNA, then lead to another transcription factors. In this study, we investigated the features and post-exercise expression patterns of two genes in the C/EBP family, C/EBPβ and C/EBPδ. The basic region leucine zipper motif (bZIP) is a crucial region of C/EBP, and analysis of synonymous and non-synonymous substitution ratios revealed that bZIP regions were well conserved in mammalian species. Quantitative real-time RT-PCR data showed that C/EBPβ and C/EBPδ expression increased significantly in the muscle after exercise, but did not do so in the blood. We therefore propose that exercise-induced increased expression of C/EBPβ and C/EBPδ may stimulate gene expression in physiological pathways in muscle cells.