한국약용작물학회지 Vol. 26 No. 4 (p.281-290)

|연구논문|
국산 한약재로 이용되는 약용식물의 NCI-H1229 인간 폐암 세포주에 대한 성장 억제효과 분석

Screening for Growth Inhibitory Effects of Medicinal Plants Used in Traditional Korean Medicine in NCI-H1229 Human Lung Cancer Cells
키워드 :
Astilbe Chinensis (Maxim.) Franch. Et Savat. Var.,Inula Japonica Thunb,Lagerstroemia Indica L.,Nelumbo nucifera Gaertn,Rubia Akane Nakai,Lung Cancer

목차

ABSTRACT
서 언
재료 및 방법
  1. 실험재료와 추출물의 제조
  2. 세포 배양
  3. MTS Assay
  4. Caspase-3 활성 측정
  5. 세포분석기 (flow cytometry)를 이용한 암세포 사멸 효과측정
  6. 통계처리
결과 및 고찰
  1. 약용식물 에탄올 추출물이 NCI-H1229 세포생존율에미치는 영향
  2. 약용식물 에탄올 추출물이 caspase-3 단백질 활성화에 미치는 영향
  3. 약용식물 에탄올 추출물의 세포사멸에 미치는 영향
  4. 약용식물 에탄올 추출물의 DNA 단편화에 미치는 영향
감사의 글
REFERENCE

초록

Lung cancer, the most common malignant disease worldwide, is the predominant cause of cancer deaths, particularly amongst men. Therefore, various researchers have focused on the growth inhibitory effects of medicinal plants used in traditional Korean medicine. This study aimed to investigate the growth inhibitory effects of ethanol extracts of Rubiae radix, Inulae flos, Nelumbinis receptaculum, Astilbe radix, and Lagerstroemia flos on NCI-H1229 cells. Method and Results: The viability of NCI-H1229 cells was evaluated in vitro using an MTS assay. Treatment with the ethanol extracts of the selected medicinal plants at 500 ㎍/㎖ reduced NCI-H1229 cell viability and increased apoptotic cell death and caspase-3 activation. In addition, treatment with ethanol extracts of Inulae flos and Astilbe radix increases DNA fragmentation, as measured by the TUNEL assay. Conclusions: These results indicated that ethanol extracts of Rubiae radix, Inulae flos, Nelumbinis receptaculum, Astilbe radix, and Lagerstroemia flos exhibited growth inhibitory effects, inducing apoptotic cell death, DNA fragmentation and caspase-3 activation in NCI-H1229 cells. Therefore, these medicinal plant extracts may be used in the development of natural medicines to inhibit the growth of lung cancers. However, further study is needed to determine the active ingredients of the ethanol extracts from medicinal plants that are reposible for the inhibitory effect on lung cancer cell grwoth.