한국약용작물학회지 Vol. 26 No. 4 (p.302-307)

|연구논문|
밤나무 근권토양에서 분리한 Ilyonectria radicicola 균주의 인삼에 대한 병원성 및 유전적 분석

Pathgenicity on Ginseng and Sequence Assays of Ilyonectria radicicola Isolated from Chestnut Rhizosphere Soils
키워드 :
Ilyonectria radicicola,Panax ginseng C. A. Meyer,Chestnut Rhizosphere Soils,Ginseng Root Rot,Weakly Virulence

목차

ABSTRACT
서 언
재료 및 방법
  1. 토양 수집 및 균주 분리
  2. 병원성 검정
  3. DNA 분리 및 염기서열을 이용한 유연관계 분석
결과 및 고찰
  1. 밤나무 근권토양의 병원균 검출
  2. 유연관계 분석을 통한 밤나무 근권토양 분리균의 종 동정
  3. 밤나무 근권 분리균의 인삼에 대한 병원성
감사의 글
REFERENCES

초록

Background: A soil-borne pathogenic fungus, Ilyonectria radicicola (Cylindrocarpon destructans) causes root rot on ginseng (Panax ginseng C. A. Meyer) and is known to attack many other plants. The Nectria/Neonectria radicicola complex has been renamed as the I. radicicola complex after analysis of its multi-gene relatedness and morphological characteristics. The fungi in this complex have been reclassified into 16 species under the genus Ilyonectria based on characteristics analysis
Methods and Results: To obtain useful data from the Korean ginseng root rot, I. radicicola was isolated from the rhizosphere soils of the chestnut tree. They were identified through a pathogenicity test and a survey of the morphological features. The existence of I. radicicola in soil samples was confirmed by PCR detections using nested PCR with species-specific primer sets. These were subsequenctly isolated on semi-selective media from PCR-positive soils. Genetic analysis of the I. radicicola complex containing these pathogens was done by comparing the DNA sequences of the histone h3 region. These isolates originating from the rhizosphere soils of chestnut constituted a clade with other closely related species or I. radicicola isolates originating from ginseng or other host plants, respectively. Additionally, the pathogenicity tests to analyze the characteristics of these I. radicicola isolates revealed that they caused weakly virulent root rot on ginseng.
Conclusions: This is the first study reporting that I. radicicola isolates from chestnut rhizosphere soils can attack ginseng plant in Korea. Thus, these results are expected to provide informations in the selection of suitable fields for ginseng cultivation.