한국약용작물학회지 Vol. 26 No. 4 (p.317-327)

|연구논문|
유화제 첨가 용융압출을 이용한 참당귀 성형체의 페놀성분 나노화 및 용해도 향상

Enhancement of Solubility and Nanonization of Phenolic Compound in Extrudate from Angelica gigas Nakai by Hot Melt Extrusion using Surfactant
키워드 :
Angelica gigas Nakai,Hot Melt Extrusion,Nano-solid Dispersion,Size Reduction,Solubility

목차

ABSTRACT
INTRODUCTION
MATERIALS AND METHODS
  1. Chemical and reagents
  2. Preparation of ultrafine powder of AGR (UFP-AGR)
  3. Preparation of chemical formulation
  4. Preparation of extrudate formulation and HMEconfiguration
  5. Particle size analysis
  6. Solubility measurements
  7. Evaluation of amorpous solid dispersion by FT-IR,DSC, and XRPD
  8. Extraction of formulation
  9. Determination of total phenolic contents (TP)
  10. Determination of total flavonoid content
  11. DPPH free radical scavenging activity
RESULTS AND DISCUSSION
  1. Particle size reduction and solubility enhancement
  2. FT-IR, DSC and XRD analysis of extrudate solidformuation of Angelica gigantis radix ultrafine powderwith chemical formulation.
  3. Total phenol content, total flavonoid content,DPPH radical scavenging activity and contents ofactive compounds in extrudate solid formuation ofAngelica gigantis radix ultrafine powder with chemicalformulation.
ACKNOWLEDGEMENTS
REFERENCES

초록

Background: The root of Angelica gigas Nakai is used as a traditional herbal medicine in Korea for the treatment of many diseases. However, the poor water solubility of the active components in A. gigas Nakai is a major obstacle to its bioavailability.
Methods and Results: This work aimed at enhancing the solubility of the active compounds of A. gigas Nakai by a chemical (using a surfactant) and physical (hot melt extrusion, HME) crosslinking method. Fourier transform infrared spectroscopy revealed multiple peaks in the case of the extrudate solids, attributable to new functional groups including carboxylic acid, alkynes, and benzene derivatives. Differential scanning calorimetry analysis showed that the extrudate soilid had a lower glass transition temperature (Tg) and enthalpy (ΔH) (Tg : 43℃, ΔH : < 6 J/g) as compared to the non-extrudate (Tg : 68.5℃, ΔH : 123.2) formulations. X-ray powder diffraction analysis revealed the amorphization of crystalline materials in the extrudate solid. In addition, enhanced solubility (53%), nanonization (403 ㎚), and a higher amount of extracted phenolic compounds were achieved in the extrudate solid than in the non-extrudate (solubility : 36%, nanonization : 1,499 ㎚) formulation. Among the different extrudates, acetic acid and span 80 mediated formulations showed superior extractions efficiency.
Conclusions: HME successfully enhanced the production of amorphous nano dispersions of phenolic compound including decursin from extrudate solid formulations.