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Inhibition of Adenovirus 36 Replication and Lipid Accumulation by Distylium racemosum KCI 등재
- 언어ENG
- URLhttps://db.koreascholar.com/Article/Detail/356347
pp.492-501
Obesity is a worldwide disease and one of the major risk factors. Virus among many factors can lead to obesity. Adenovirus 36 (Ad-36) is the adipogenic virus linked with human obesity. Nevertheless, there is no drug to treat both Ad-36 infection and obesity associated with virus. For the precedent study on anti-cholesterol test, Distylium racemosum (D. racemosum), Quercus salicina (Q. salicina) and Raphiolepis indica (R. indica) were selected. This study was carried out to evaluate the anti-cholesterol effects, anti-lipid effects and inhibition of Ad-36 replication from three extracts. D. racemosum (50 μg/mL) inhibited lipid accumulation on 3T3-L1 adipocyte. D. racemosum inhibited adipocyte differentiation through suppression of regulator peroxisome proliferator-activated receptor- γ (PPARγ) genes and adipocyte-specific genes such as adipocyte protein 2 (aP2). D. racemosum inhibited replication of Ad-36 at 50 μg/mL of concentration. Therefore, the extract of D. racemosum could be a candidate for development of anti-Ad-36 and anti-obesity drugs.
1. Introduction
2. Materials and Methods
2.1. Extraction of medicinal plants
2.2. Cholesterol adsorptivity
2.3. Cell viability assays
2.4. Differentiation of 3T3-L1preadipocytes
2.5. Oil Red O staining of 3T3-L1adipocytes
2.6. Reverse transcription-PCR (RT-PCR)
2.7. Inhibition of Adenovirus 36 replication
2.8. Statistical analysis
3. Results and Discussion
3.1. Cholesterol reduction effects of thenatural extracts
3.2. Cell viability of the natural extractson 3T3-L1 and A549 cells
3.3. Inhibitory effect on adipogenesis andlipid accumulation by the naturalextracts
3.4. Inhibition of adipogenic specificgenes expression by the naturalextracts
3.5. Inhibitory effects of Ad-36replication by the natural extracts
4. Conclusion
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