환경생물 Vol. 36 No. 3 (p.424-437)

|Original article|
Seasonal Changes in Cyanobacterial Diversity of a Temperate Freshwater Paldang Reservoir (Korea) Explored by using Pyrosequencing

키워드 :
cyanobacterial diversity,Paldang Reservoir,Han River,pyrosequencing,16S rRNA

목차

Abstract
INTRODUCTION
MATERIALS AND METHODS
  1. Environmental factors and water sample collections
  2. Nutrient data and chlorophyll a measurement
  3. Environmental DNA extraction
  4. PCR and 454 pyrosequencing
  5. Data cleaning and BLAST (Basic Local Alignment Search Tool) searches
  6. Phylogenetic and statistical analyses
RESULTS AND DISCUSSION
  1. Environmental conditions and phytoplanktons in the Paldang Reservoir
  2. Pyrosequence characteristics and taxonomic affiliations
  3. Phylogenetic analysis of cyanobacterial community
  4. Cyanobacterial distribution pattern - pyrosequencing and microscopic data
  5. Implications of molecular cyanobacterial detection
REFERENCES

초록

The incidence of freshwater algal bloom has been increasing globally in recent years and poses a major threat to environmental health. Cyanobacteria are the major component of the bloom forming community that must be monitored frequently. Their morphological identities, however, have remained elusive, due to their small size in cells and morphological resemblances among species. We have analyzed molecular diversity and seasonal changes of cyanobacteria in Paldang Reservoir, Korea, using morphological and 16S rRNA pyrosequencing methods. Samples were collected at monthly intervals from the reservoir March-December 2012. In total, 40 phylotypes of cyanobacteria were identified after comparing 49,131 pyrosequence reads. Cyanobacterial genera such as Anabaena, Aphanizomenon, Microcystis and Synechocystis were predominantly present in samples. However, the majority of cyanobacterial sequences (65.9%) identified in this study were of uncultured origins, not detected morphologically. Relative abundance of cyanobacterial sequences was observed as high in August, with no occurrence in March and December. These results suggested that pyrosequencing approach may reveal cyanobacterial diversity undetected morphologically, and may be used as reference for studying and monitoring cyanobacterial communities in aquatic environments.