This study was conducted to compare anti-inflammatory effect of Robinia pseudoacacia L. using different extraction methods (water extraction, ethanol extraction and high temperature extraction). We investigated anti-inflammatory effect of Robinia pseudoacacia L. extract (RP1, water extract; RP2, ethanol extract; RP3, high temperature extract) on lipopolysaccharide (LPS)-stimulated inflammation using Raw 264.7 cell. Cells were treated with various concentrations (12.5, 25, 50, 100 or 200 ㎍/㎖) of water extract, ethanol extract and high temperature extract. Cytotoxicity was not observed on Raw 264.7 cells, LPS-stimulated production of NO (nitric oxide), PGE2 (prostaglandin E2) and cytokines (TNF-α, IL-6 and IL-1β) was reduced by RP3 treatment more than RP1 and RP2. In conclusion, these results indicated that inflammation on Raw 264.7 cells was improved by RP3. Treatment of RP3 could be used to natural medicine for improving inflammatory response. However, further experiment is required to observe how the high temperature extraction at 500℃ for 48 h influences on alteration of active ingredient in Robinia pseudoacacia L., and conducts the inflammation signal pathway on Raw 264.7 cells.

Abstract
 서 언
 재료 및 방법
  실험 재료와 추출물의 제조
  세포배양
  세포생존율 분석
  NO (nitric oxide) 농도 측정
  Prostaglandin E2 (PGE2) 농도 측정
  Cytokines (TNF-α, IL-1β, IL-6) 농도 측정
  통계처리
 결과 및 고찰
  추출물의 Raw 264.7 세포에 대한 세포독성 평가
  추출물의 NO(nitric oxide)생성 억제효과 분석
  추출물의 PGE2 생성 억제효과 분석
  추출물의 TNF–α 생성 억제효과 분석
  추출물의 IL-6 생성 억제효과 분석
  추출물의 IL-1β 생성 억제효과 분석
 적 요
 References

• 노종현(한약진흥재단) | Jong Hyun Nho (National Development Institute of Korean Medicine)
• 강병만(한약진흥재단) | Byoung Man Kang (National Development Institute of Korean Medicine)
• 정원석(한약진흥재단) | Won Seok Jung (National Development Institute of Korean Medicine) 교신저자