In this study, a preliminary evaluation of the antioxidant and anti-inflammatory activity of the Ficus erecta var. sieboldii (Miq.) King (FES) leaf extract has been performed to assess its potential as a natural resource for food and medicinal materials. FES was extracted using 70% EtOH and then fractionated sequentially using n-hexane, CH2Cl2, EtOAc, and n-BuOH. To screen for antioxidant and anti-inflammatory agents effectively, the inhibitory effect of the FES extracts on the production of oxidant stresses (DPPH, xanthine oxidase, and superoxide) and pro-inflammatory factors (NO, iNOS, COX-2, PGE2, IL-6, and IL-β) in the murine macrophage cell line RAW 264.7 activated with lipopolysaccharide (LPS) was examined. Among the sequential solvent fractions of FES, the CH2Cl2 and EtOAc fractions showed decreased production of oxidant stresses (DPPH, xanthine oxidase and superoxide), and the hexane and CH2Cl2 fractions of FES inhibited the production of pro-inflammatory factors (NO, iNOS, COX-2, and PGE2). The CH2Cl2 fraction also inhibited the production of pro-inflammatory cytokines (TNF-α, IL-6, and IL-1β). These results suggest that FES has a significant effects on the production of oxidant stresses and pro-inflammatory factors and may be used a natural resource for antioxidant and anti-inflammatory agents.

Abstract
 서 언
 재료 및 방법
  시료의 추출
  DPPH radical 소거활성에 의한 항산화활성 검색
  Xanthin oxide 억제 및 superoxide 소거활성 검색
  세포 배양
  Nitric oxide (NO) assay
  세포독성 평가(LDH assay)
  prostaglandin E2 (PGE2) 생성 억제 효능 평가
  염증성 사이토카인(TNF-α, IL-6, IL-1β) 생성 억제 효능평가
  Western blot analysis
  통계분석
 결과 및 고찰
  DPPH radical 소거활성에 의한 항산화 활성 검색
  Xanthine oxidase 억제
  superoxide 소거 활성
  세포 독성에 미치는 영향
  Nitric oxide 생성억제 효과
  Pro-inflammatory cytokine 생성 억제 효과
  Prostagladin E2 (PGE2) 생성에 미치는 영향
  iNOS 생성에 미치는 영향
  COX-2 발현에 미치는 영향
 적 요
 References

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