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코끼리마늘(Allium ampeloprasum L.)의 기내 미숙총포 배양을 통한 다신초유도와 종구대량증식 시스템 KCI 등재

Multiple Shoot Induction and Bulb Mass Proliferation System by in Vitro Immature Spathe Culture of Elephant Garlic (Allium ampeloprasum L.)

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한국자원식물학회지 (Korean journal of plant resources)
한국자원식물학회 (The Plant Resources Society Of Korea)
초록

This study was performed to develop the mass propagation system using tissue culture technique to supply the seeds of Elephant garlic (Allium ampeloprasum L.) which has difficulty in propagation. Immature spathe of Elephant garlic was cultured on Murashige & Skoog (MS) medium supplemented with two plant growth regulators, naphthaleneacetic acid (NAA) and kinetin. After 6 weeks of culture, the highest number of shoot (14.9/explant) was obtained when the immature spathe with 10 ㎝ length was cultured right after harvesting. In MS medium supplemented with 2 ㎎/L kinetin and 0.5 ㎎/L NAA, the most vigorous growth characteristics was observed, the shoot number was 14.9/explant, its length was 11.3 ㎝, and its fresh weight was 2.5 g. When the bulblets were cultured in MS medium with 2 ㎎/L kinetin and 0.5 ㎎/L NAA, the addition of 30 ㎎/L adenine improved their proliferation and growth significantly, the highest bulblet formation rate (48%) was obtained. The addition of 7% sucrose also increased the bulblet formation rate at the highest frequency of 98.2%. The shoots were shown be more vigorously proliferated at the secondary subculture stage rather than primary culture stage, their propagation rate was 80% after subculture.

목차
Abstract
 서 언
 재료 및 방법
  식물재료
  다신초 배양
  기내소구 배양
  통계처리
 결과 및 고찰
 적 요
 References
저자
  • 권영희(충청북도농업기술원) | Young Hee Kwon (Chungcheongbuk-do Agricultural Research & Extension Services) 교신저자
  • 정재현(충청북도농업기술원) | Jae Hyun Jeong (Chungcheongbuk-do Agricultural Research & Extension Services)
  • 이재선(충청북도농업기술원) | Jae Sun Lee (Chungcheongbuk-do Agricultural Research & Extension Services)
  • 전종옥(충청북도농업기술원) | Jong Ok Jeon (Chungcheongbuk-do Agricultural Research & Extension Services)
  • 박영욱(충청북도농업기술원) | Young Uk Park (Chungcheongbuk-do Agricultural Research & Extension Services)
  • 민지현(충청북도농업기술원) | Ji Hyun Min (Chungcheongbuk-do Agricultural Research & Extension Services)
  • 장후봉(충청북도농업기술원) | Who Bong Chang (Chungcheongbuk-do Agricultural Research & Extension Services)
  • 이상영(충청북도농업기술원) | Sang Young Lee (Chungcheongbuk-do Agricultural Research & Extension Services)
  • 윤철구(충청북도농업기술원) | Cheol Ku Youn (Chungcheongbuk-do Agricultural Research & Extension Services)
  • 김기현(충청북도농업기술원) | Ki Hyun Kim (Chungcheongbuk-do Agricultural Research & Extension Services)