Pesticide application in agriculture provides significant benefits such as protection from disease, prevention of harmful insects, and increased crop yields. However, accurate toxicological tests and risk assessments are necessary because of many related adverse effects associated with pesticide use. In this review, we discuss and analyze residual pesticides contained in livestock feed in Korea. A pesticide residue tolerance standard for livestock feed has not been precisely established; so, risk assessments are required to ensure safety. Standards and approaches for animal criteria and appropriate methods for evaluating residual pesticides are discussed and analyzed based on technology related to animal product safety in Korea. The safety of livestock feed containing pesticides is assessed to establish maximum residue limits relative to pesticides. Analysis of residual pesticides in milk, muscle, brain, and fat was performed with a livestock residue test and safety evaluation of the detected pesticide was performed. Efficacy of organic solvent extraction and clean-up of feed was verified, and suitability of the instrument was examined to establish if they are effective, rapid, and safe. This review discussed extensively how pesticide residue tolerance in livestock feed and hazard evaluation may be applied in future studies.

ABSTRACT
 Ⅰ. INTRODUCTION
  1. Positive list system (PLS) in food and feedstuffs
  2. Major contaminations in agricultural commodities
  3. Pesticides application lists in feed
  4. Pesticide maximum residue limits (MRLs)
  5. Suitable analytical methodologies
  6. Pesticide residue testing of livestock products
  7. Risk assessment of dietary intake in feed-based pesticides
  8. Conclusions and future perspectives
 Ⅱ. CONFLICT OF INTEREST
 Ⅲ. ACKNOWLEDGEMENTS
 Ⅳ. RERERENCES

• Jin Young Jeong(Animal Nutrition & Physiology Team, National Institute of Animal Science)
• Minseok Kim(Animal Nutrition & Physiology Team, National Institute of Animal Science)
• Youl-Chang Baek(Animal Nutrition & Physiology Team, National Institute of Animal Science)
• Jaeyong Song(Animal Nutrition & Physiology Team, National Institute of Animal Science)
• Seul Lee(Animal Nutrition & Physiology Team, National Institute of Animal Science)
• Ki Hyun Kim(Animal Nutrition & Physiology Team, National Institute of Animal Science)
• Sang Yun Ji(Animal Nutrition & Physiology Team, National Institute of Animal Science)
• Hyun-Jeong Lee(Animal Nutrition & Physiology Team, National Institute of Animal Science)
• Young Kyun Oh(Animal Nutrition & Physiology Team, National Institute of Animal Science)
• Sung Dae Lee(Animal Nutrition & Physiology Team, National Institute of Animal Science) Corresponding author