천연물 유래 단일 성분의 지방세포로의 분화 및 지방축적 억제효과에 대한 잠재성을 탐색하기 위해 본 연구에서는 화살나무의 껍질에서 분리 정제한 FG가 3T3-L1 지방전구세포에 있어서 지방세포로의 분화 및 지방구 축적에 미치는 영향에 대해 평가하고, 관련 메카니즘에 대해 검토하였다.
FG는 분화용 배지에 포함된 분화 촉진인자들의 자극에 의한 지방생성에 있어 주요한 전사인자인 PPARγ와 그 표적 단백질인 FABP4의 발현을 억제함으로써 지방세포 분화 및 지방구 축적을 억제하는 것으로 나타났다. 이상의 결과로부터 FG가 비만이나 비만과 관련된 당뇨병 등을 예방, 개선하는데 유용한 물질로 사용될 가능성이 있을 것으로 생각된다.

Obesity is the most common metabolic disease and is caused by an excess accumulation of body fat. 1-O-Feruloyl-β-D-glucose (FG) is a phenolic compound isolated from the corks of Euonymus alatus (Thunb.) Sieb. This study was designed to investigate the effect of FG on adipogenesis using 3T3-L1 preadipocyte. First, we evaluated the effects of FG on cell proliferation and cytotoxicity. Up to 5 μM, FG did not affect the proliferation of or cause cytotoxicity in 3T3-L1 preadipocytes. Therefore, we used FG at 0-5 μM for subsequent experiments. FG inhibited the accumulation of lipid droplets in differentiated 3T3-L1 adipocytes in a concentration-dependent manner. To understand the mechanism underlying the anti-adipogenic effect of FG, western blot analyses were performed. We found that FG suppressed peroxisome proliferator-activated receptor (PPAR) γ protein expression, a representative adipogenesis-related transcription factor. In addition, FG inhibited aP2/fatty acid binding protein 4 (FABP4) protein expression, a target gene of PPARγ. In conclusion, these data suggest that FG inhibits adipogenesis in 3T3-L1 adipocytes by inhibiting the expression of PPARγ and FABP4.

• Sang Hee Kwak(Department of Food and Nutrition, College of Engineering, Daegu University) | 곽상희
• Yoon Hee Kim(Department of Food and Nutrition, College of Engineering, Daegu University, Research Institute of Anti-aging, Daegu University, Institute of Industrial and Technology, Daegu University) | 김윤희 Corresponding author