산업식품공학 제22권 제4호 (p.309-314)

당근, 느타리버섯 및 솔잎 추출물이 첨가된 화장품의 할랄인증을 위한 PCR 분석

PCR Analysis for Halal Authentication of Cosmetics Containing Carrot, Oyster Mushroom, and Pine Needle Extracts
키워드 :
halal food,haram,PCR,plant extracts,rapid detection

목차

Abstract
서 론
재료 및 방법
  식물 추출물과 화장품
  샘플에서 chromosomal DNA 및 template DNA의 추출
  Oligonucleotide primers
  PCR
  Template DNA의 copy number 결정
결과 및 고찰
  Template DNA의 copy number
  PCR 방법의 검출한계
  추출물 및 추출물이 포함된 화장품에 PCR 방법의 적용
요 약
References

초록

In recent years, interest in halal authentication from the domestic food and cosmetics field has been growing for advances into the overseas halal market. For halal authentication, the product must not contain haram ingredients derived from pig, dog, human, GMO, etc. In this study, the presence of haram ingredients in plant extracts (carrot, oyster mushroom, and pine needle) treated with papain and bromelain and cosmetics (mask pack and cream) containing these extracts were analyzed by PCR to confirm whether these cosmetics were suitable for halal authentication. Detection limits of the PCR method that specifically detected template DNA of human, pig, dog, and GMO were 1.29×103, 1.14×103, 1.24×102 and 2.02×103 copies/tube, respectively. PCR was not inhibited by the plant extracts or cosmetic ingredients. Results of PCR for the plant extracts or cosmetics containing these extracts were all negative. This PCR method could be used to rapidly identify the presence of haram ingredients in raw materials or final products during the manufacturing process of food and cosmetics.