We analyzed the nuclear ribosomal internal transcribed spacer (ITS) sequence of common buckwheat, Fagopyrum esculentum and tartary buckwheat, F. tataricum. The diversity of the nucleotides and haplotypes, Tajima’s D, and Fu’s Fs was analyzed and compared among the varieties of common buckwheat and tartary buckwheat. The diversity of nucleotides and haplotypes indicated that the buckwheat populations had undergone rapid population expansion but D and Fs did not support their expansion statistically. The phylogenetic analysis of ITS sequences did not clearly establish the phylogenetic relationships between the varieties of common buckwheat. The In/Del sequence of ITS-1 region could, therefore, be used as a DNA marker to distinguish raw or manufactured products derived from common buckwheat and tartary buckwheat.

Ⅰ. 서 론
 Ⅱ. 재료 및 방법
  1. 연구재료
  2. DNA 추출 및 염기서열 확보
  3. 염기서열 분석
  4. 유연관계 분석 및 In/Del 마커
 Ⅲ. 결과 및 고찰
  1. ITS 염기서열 분석
  2. 메밀 품종간 계통유연관계
  3. Insertion-deletion (In/Del) 마커
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