1. 본 연구는 한국 재래종 강낭콩 209자원의 phytochemical 및 항산화활성을 평가하였다.
2. 항산화활성은 DPPH, ABTS, FRAP, SOD를 분석하였으며 phytochemical은 kaempferol, myricetin, quercetin, naringenin 함량을 각각 분석하였다.
3. 항산화활성은 강낭콩 자원 간 다양한 분포를 보였으며 DPPH의 경우 62.3~643.9 (IC50), ABTS의 경우 0.28~1.49 mgAAE/g, FRAP의 경우 0.41~5.44 mgAAE/g, SOD의 경우 50.4 ~ 299.8 (IC50)로 나타났다.
4. Relative antioxidant capacity index (RACI)로 강낭콩 자원의 항산화활성을 비교한 결과 IT104587이 가장 높은 항산화활성을 보였으며 IT189598이 가장 낮은 항산화활성을 보였다.
5. 분석된 Phytochemical 중에서 한국 재래종 강낭콩에서는 Kaempferol이 가장 높은 함량을 나타냈다.
6. PCA 분석 결과 209자원은 3개의 그룹으로 나뉘었으며 이중 그룹 III에 속한 46자원의 강낭콩이 낮은 항산화활성 및 phytochemical 함량을 보였다.
7. 본 연구 결과는 한국 재래종 강낭콩의 항산화활성 및 phytochemical 정보를 제공하며 이 정보는 강낭콩 품종 개발을 위한 기초 정보로 사용될 수 있을 것이다.

The Korean common bean (Phaseolus vulgaris L.) has been receiving increased attention as a functional food. The objective of this study was to reveal the phytochemicals genetic variation and antioxidant activity of 209 Korean common bean landraces. Antioxidant activity was evaluated with the DPPH (2,2-diphenyl-1-picryl-hydrazyl-hydrate), ABTS (2,2’-azino-bis(3-ethylbenzothiazoline-6-sulphonic acid), ferric reducing antioxidant power (FRAP), and superoxide dismutase (SOD) assay. Antioxidant activities among common bean accessions showed wide variation. Four flavonoids (kaempferol, myricetin, quercetin, and naringenin) of the 209 Korean common bean landraces were measured using HPLC. Among them, kaempferol had the highest phytochemicals compared to the other three flavonoids. Using the relative antioxidant capacity index (RACI), it was found out that the IT104587 had the highest antioxidant activity. Meanwhile, in clustering analysis, the Korean common bean landraces were classified into three clusters. Among them, cluster II contained 64 landraces with higher antioxidant activities and phytochemicals than the other clusters, except DPPH. The results could provide information on the valuable Korean common bean landraces for the development of new common bean varieties.

ABSTRACT
 Introduction
 Materials and Methods
  Plant materials
  Common bean extraction
  DPPH assay
  ABTS assay
  Ferric reducing antioxidant power assay (FRAP)
  SOD assay
  Total polyphenol content assay (TPC)
  Total flavonoid content assay (TFC)
  Analysis of flavonol content in Korean commonbean landraces
  Data analysis
 Results
  Phytochemicals contents in 209 Korean commonbean landraces
  Antioxidant activities of 209 Korean common beanlandraces
  Correlation among the phytochemicals and antioxidantactivities of the 209 Korean common bean landraces
  Principal Component Analysis (PCA)
 Discussion
 적 요
 REFERENCES

• Kyung Jun Lee(National Agrobiodiversity Center, National Institute of Agricultural Sciences (NAS), RDA) | 이경준
• Myoung-Jae Shin(National Agrobiodiversity Center, National Institute of Agricultural Sciences (NAS), RDA) | 신명재
• Gyu-Taek Cho(National Agrobiodiversity Center, National Institute of Agricultural Sciences (NAS), RDA) | 조규택
• Gi-An Lee(Research Policy Bureau, RDA) | 이기안
• Kyung-Ho Ma(Department of Herbal Crop Research, National Institute of Horticultural and Herbal Science (NIHHS), RDA) | 마경호
• Jong-Wook Chung(Department of Industrial Plant Science & Technology, Chungbuk National University) | 정종욱
• Jung-Ro Lee(National Agrobiodiversity Center, National Institute of Agricultural Sciences (NAS), RDA) | 이정로 Corresponding author