한국환경과학회지 제28권 제2호 (p.249-257)

|ORIGINAL ARTICLE|
소장상피세포에 있어서 느릅나무 당단백질이 톨루엔에 의해 유도된 환경독성 기작에 미치는 효과

Effects of a Glycoprotein Isolated from Ulmus davidiana Nakai on Toluene-Induced Ecotoxicity and its Mechanism in Human Intestinal Epithelial Cells
키워드 :
Ulmus davidiana Nakai (UDN) glycoprotein,Intestinal epithelial cells,Reactive oxygen species,NF-κB,Toluene

목차

Abstract
1. 서 론
2. 재료 및 실험방법
3. 결과 및 고찰
  3.1. 느릅나무 당단백질의 환경독성 제어효과
  3.2. 활성산소종 생성 억제를 통한 느릅나무 당단백질의환경독성 제어효과
  3.3. p38 MAPK의 인산화 억제를 통한 느릅나무당단백질의 환경독성 제어효과
  3.4. NF-κB의 인산화 억제를 통한 느릅나무 당단백질의환경독성 제어효과
  3.5. 느릅나무 당단백질의 환경독성 세포신호전달 기전제어효과
4. 결 론
REFERENCE

초록

Ulmus davidiana Nakai (UDN) has been traditionally used as a herbal medicine to treat inflammatory diseases in Korea. In the present study, we investigated the anti-ecotoxic potential of a 116 kDa glycoprotein isolated from UDN (UDN glycoprotein) in human intestinal epithelial INT-407 cells. We demonstrated that UDN glycoprotein (20 μg/mL) could inhibit the production of lactate dehydrogenase (LDH) induced by toluene, an ecotoxic substance. Additionally, we found that the toluene-induced intestinal cytotoxicity was mediated by the phosphorylation of p38 Mitogen-Activated Protein Kinase (MAPK) via the production of intracellular Reactive Oxygen Species (ROS). The UDN glycoprotein significantly decreased the levels of ROS production and p38 MAPK activation in toluene-stimulated INT-407 cells. Moreover, the UDN glycoprotein inhibits the phosphorylation of nuclear factor-kappa B (NF-κB), which is responsible for the production of LDH, in toluene-stimulated INT-407 cells. Collectively, our data indicate that UDN glycoprotein is a natural antioxidant and a modulator of ecotoxicity signaling pathways in human intestinal epithelial cells.