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복합기능성 Bacillus velezensis GH1-13 균주의 대량배양 최적화 및 특성 KCI 등재

Mass Cultivation and Characterization of Multifunctional Bacillus velezensis GH1-13

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한국유기농업학회지 (Korea Journal of Organic Agriculture)
한국유기농업학회 (Korea Association Of Organic Agriculture)
초록

Bacillus genus are found abundantly in various sites and their secondary metabolites were used as potential agents in agriculture, notably plant growth promoting and bio-control. The objective of this study was to develop the culture conditions of GH1-13 strain including higher cell growth, stable endospore-forming and enhancement of potential agents which are related with plant growth promoting and phytopathogen suppression. The optimal carbon and nitrogen sources were determined by glucose and soy bean flour, respectively, then resulted in 7.5×109 cells/mL, 6.8×109 endospore cells/mL and sporulation yield of 90% after 30 h cultivation in 500 L submerged fermenter at 37℃, pH 7.0. Cells and cell-free supernatant of GH1-13 strains showed the potent antifungal activity against phytopathogenic fungi of Colletotrichum gloeosporioides. It was also confirmed that indole-3-acetic acid (IAA) production of GH1-13 strain was greatly increased by addition of 0.3% tryptophan.

목차
Ⅰ. 서 론
 Ⅱ. 재료 및 방법
  1. 균주 및 배양 조건
  2. 최적 탄소원 및 질소원 선발
  3. 배지 최적화 및 균주 배양
  4. 최적배지를 이용한 대량배양
  5. 식물 병원성 곰팡이에 대한 항진균활성 검정
  6. Indole-3-acetic acid (IAA) 생산성
 Ⅲ. 결과 및 고찰
  1. 대량배양용 배지조성 최적화
  2. 최적배지를 이용한 대량배양 조건 확립
  3. 식물 병원성 곰팡이에 대한 항진균활성 검정
  4. IAA 최대 생산을 위한 배양기술 확립
 Ⅳ. 적 요
 References
저자
  • 박준경((재)농축산용미생물산업육성지원센터) | Park Jun-Kyung
  • 김주은(전남대학교 자연과학대학 화학과) | Kim JuEun
  • 이철원(전남대학교 자연과학대학 화학과) | Lee Chul-Won
  • 송재경(국립농업과학원 농업생물부 농업미생물과) | Song JaeKyeong
  • 서선일((재)농축산용미생물산업육성지원센터) | Seo Sun-Il
  • 봉기문(국립환경과학원 영산강물환경연구소) | Bong Ki-Moon
  • 김대혁((재)농축산용미생물산업육성지원센터, 전북대학교 자연과학대학 분자생물학과) | Kim Dae-Hyuk
  • 김평일((재)농축산용미생물산업육성지원센터) | Kim Pyoung Il Corresponding author