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pp.173-182
The bacterial strains, which utilizes 2,4,4`-trichloro-2`-hydroxydiphenyl ether(TCHDPE) as a sole carbon source, were isolated by selective enrichment culture from soil samples of industrial waste deposits. The bacterium that showed the highest biodegradation activity was designated as EL-047R. The isolated strain EL-047R was identified as the genus Pseudomonas from the results of morphological, cultural, and biochemical tests. The optimum conditions of medium for the growth and the degradation of TCHDPE were TCHDPE 500 ppm, (NH_4)_2SO_4 0.1% as the nitrogen source, initial pH 7.0±0.1, and 37℃, respectively. In this conditions, the degradation rate of TCHDPE was about 97%. Pseudomonas sp. EL-047R was tested for resistance to several metal compounds and antibiotics. Pseudomonas sp. EL-047R was moderately grown to Cd(NO_3)_2, ZnCl_2, AgSO_4, CuSO_4 and HgCl_2. This strain was sensitive to rifampicin and kanamycin but resistant to ampicillin, penicillin, tetracyclin and chloramphenicol. Pseudomonas sp. EL-047R was grown structurally related compounds and potential metabolites of TCHDPE, and has the stability on TCHDPE biodegradation.
