An efficient shoot regeneration condition for pea cv. ‘Sparkle’ was developed by using optimum explant, plant growth regulator concentrations, and pretreatment of BA onto explant. The average shoot number per explant showed the highest on two kinds of shoot induction media (MSB5 media containing 2 ㎎/L BA and a combination of 2 ㎎/L BA and 1 ㎎/L TDZ) when cotyledonary node explants were cultured. Moreover, the pretreatment of explant in 200 ㎎/L BA solution was found to be more effective in shoot induction than that of non-pretreatment. By histological study, cell division and proto-meristem were formed near the surface of the sub-epidermal and epidermal cell layers of cotyledonary node in earlier than 3 days after culture. The analysis of genetic stability of regenerants by using thirteen ISSR markers showed that in vitro regenerated plants showed polymorphism with 8.3% compared with their mother plants.

Abstract
 Introduction
 Materials and Methods
  Plant materials
  Media preparation, culture conditions and shoot regeneration frequency
  Pretreatment of plant growth regulator (PGR) and selection of explant types
  Histological study on shoot formation
  Genomic DNA extraction and qualification
  DNA amplification and ISSR analysis
 Results and Discussion
  The optimizing plant growth regulators for shoot regeneration
  Effect of BA pretreatment and types of explant segment for shoot induction
  Histological study on shoot formation
  Genetic stability of regenerants by ISSR markers
 References

• Vipada Kantayos(School of Plant Production Science, Sunchon National University)
• Chang-Hyu Bae(School of Plant Production Science, Sunchon National University, Department of Well-being Resources, Sunchon National University) Corresponding author