A gram-positive bacterium was isolated from the spent substrate of Agaricus bisporus that showed a marked antagonistic activity against Pseudomonas agarici. It was identified as Bacillus safensis HC42 based on its cultural, biochemical, and physiological characteristics, and 16S rRNA sequence. B. safensis HC42 was saprophytic, but not parasitic or pathogenic, in cultivated mushrooms and showed strong inhibition of P. agarici in vitro. Moreover, it showed a control efficacy of 66 % against browning disease caused by P. agarici in Agaricus bisporus. Therefore, B. safensis HC42 may be useful in the future for the development of a biocontrol system.

ABSTRACT
 서 론
 재료 및 방법
  미생물 분리
  길항미생물 HC42의 선발
  길항미생물 HC42의 DNA 분리 및 16S rRNA 유전자염기서열의 결정
  길항미생물 HC42의 생리·생화학적 특성
  길항미생물 HC42의 방제효과 검정
 결과 및 고찰
  길항미생물 HC42의 항균효과
  길항미생물 HC42의 생리·생화학적인 특성
  길항미생물 HC42의 생물 검정 효과
 적 요
 감사의 글
 References

• 이찬중(농촌진흥청 국립원예특작과학원 버섯과) | Chan-Jung Lee (Mushroom Research Division, NIHHS, RDA) Corresponding author
• 이은지(농촌진흥청 국립원예특작과학원 버섯과) | Eun-Ji Lee (Mushroom Research Division, NIHHS, RDA)
• 박혜성(농촌진흥청 국립원예특작과학원 버섯과) | Hae-Sung Park (Mushroom Research Division, NIHHS, RDA)
• 공원식(농촌진흥청 국립원예특작과학원 버섯과) | Won-Sik Kong (Mushroom Research Division, NIHHS, RDA)