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남해안에서 분리한 유독 와편모조류 Gymnodinium catenatum Graham (Dinophyceae): 형태, 분자계통학적 특성 및 온도와 염분에 따른 성장 특성 KCI 등재

Toxic dinoflagellate Gymnodinium catenatum Graham (Dinophyceae) from the southern coast of Korea: morphology, phylogeny and effects of temperature and salinity on growth

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  • URLhttps://db.koreascholar.com/Article/Detail/367958
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한국환경생물학회 (Korean Society Of Environmental Biology)
초록

The toxic dinoflagellate Gymnodinium catenatum isolated from the southern coast of Korea was described under light and scanning electron microscopy, and its large subunit (LSU) rDNA was sequenced. In addition, the effects of temperature and salinity on its growth were investigated. The cells of G. catenatum, as viewed under the electronic microscope, were green-brown color, 38.1-77.4 μm in length and 26.1-40.8 μm in width. The epicone was conical, while the hypocone was trapezoidal. The nucleus was located at the central part of the cell. The apical groove was horseshoe-shaped and small pores were irregularly distributed on the cell surface. Molecular phylogeny based on LSU rDNA gene sequences showed that the Korean G. catenatum and previously reported species formed a monophyletic clade within Gymnodinium sensu stricto clade. The maximum growth rate of 0.37 day-1, was obtained at 25°C and 35 psu, and the maximum cell density of 1,073 cells mL-1, was observed at 20°C and 25 psu. However, G. catenatum did not grow at temperature <15°C and <30°C. These results suggest that environmental conditions of summer and autumn in the southern coast of Korea may be favorable for the growth of G. catenatum.

목차
Abstract
 서 론
 재료 및 방법
  1. Gymnodinium catenatum 분리 및 배양
  2. Gymnodinium catenatum 의 형태적 관찰
  3. Genomic DNA 추출, PCR 증폭 및 DNA 염기서열분석
  4. 분자계통학적 분석
  5. 온도 및 염분 조건에 따른 성장 변화
 결과 및 고찰
  1. Gymnodinium catenatum 의 형태적 특징
  2. Gymnodinium catenatum 의 계통 분류학적 특성
  3. 온도와 염분 변화에 따른 Gymnodiniumcatenatum 의 성장 특성
 적 요
 사 사
 REFERENCES
저자
  • 한경하(한국해양과학기술원 해양시료도서관) | Kyong Ha Han (Library of Marine Samples, Korea Institute of Ocean Science and Technology)
  • Li Zhun(한국해양과학기술원 해양시료도서관) | Zhun Li (Library of Marine Samples, Korea Institute of Ocean Science and Technology)
  • 강병준(한국해양과학기술원 해양시료도서관/부경대학교 해양학과,) | Byeong Jun Kang (Library of Marine Samples, Korea Institute of Ocean Science and Technology/Department of Oceanography, Pukyong University)
  • 윤주연(한국해양과학기술원 해양시료도서관) | Joo Yeon Youn (Library of Marine Samples, Korea Institute of Ocean Science and Technology)
  • 신현호(한국해양과학기술원 해양시료도서관) | Hyeon Ho Shin (Library of Marine Samples, Korea Institute of Ocean Science and Technology) Corresponding author