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뽕나무(Morus alba)와 꾸지뽕나무(Cudrania tricuspidata)의 부위에 따른 항산화 활성 및 3T3-L1세포 지방축적 억제 효과 KCI 등재

Antioxidative Activities and Inhibitory Effects on Lipid Accumulation of Extracts from Different Parts of Morus alba and Cudrania tricuspidata

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한국식품영양학회지 (The Korean Journal of Food And Nutrition)
한국식품영양학회 (The Korean Society of Food and Nutrition)
초록

In this study, we examined antioxidative effects and the anti-adipogenesis effect of different parts of Cudrania tricuspidata (C), and Morus alba (M). Total polyphenol contents were highest in M-root (34.56±0.045 mg GAE/g), and there was no significant difference, between C-root and M-leaf. Total flavonoid contents of C-root were highest (23.07±0.004 mg QE/g). To examine antioxidant activities of C and M extracts, DPPH and ABTS radical scavenging activity, and FRAP assay, was used. Results show that antioxidant activities of C and M extracts increased, in a dose-dependent manner. Adipocytes are generated by preadipocyte differentiation, during adipogenesis. Matured adipocytes accumulate in abnormal and cause obesity. We investigated effects of leaf and root extracts of C and M, on lipid accumulation, in 3T3-L1 adipocytes. Changes in cell morphology, and degrees of lipid accumulation in adipocytes, were evaluated by Oil Red O staining. Root extracts of C and M, reduced lipid content in a dose-dependent manner. Therefore, root extracts of C and M, may be good candidates for managing obesity.

목차
Abstract
 서 론
 재료 및 방법
  1. 실험재료
  2. 추출물 제조
  3. 총 페놀 함량 측정(Total phenolic content: TPC)
  4. 총 플라보노이드 함량 측정(Total flavonoid content:TFC)
  5. DPPH 라디칼 소거능
  6. ABTS 라디칼 소거능
  7. 환원력(FRAP) 측정
  8. 3T3-L1 지방전구세포 배양 및 분화
  9. MTT assay
  10. Oil Red O 염색
  11. 통계처리
 결과 및 고찰
  1. 추출 수율과 총 페놀 및 플라보노이드 함량
  2. DPPH 라디칼 소거능
  3. ABTS 라디칼 소거능
  4. 환원력(FRAP) 측정
  5. 꾸지뽕나무와 뽕나무의 잎과 뿌리 추출물의 지방세포내 독성평가
  6. 지방전구세포 내 지질생성에 대한 꾸지뽕나무와 뽕나무의 잎과 뿌리 추출물의 효과
 요약 및 결론
 References
저자
  • 김건희(덕성여자대학교 식품영양학과) | Gun-Hee Kim (Dept. of Food and Nutrition, Duksung Women’s University) Corresponding author
  • 김은향(덕성여자대학교 식품영양학과) | Eunhyang Kim (Dept. of Food and Nutrition, Duksung Women’s University)