한국약용작물학회지 Vol. 27 No. 1 (p.24-29)

HPLC에 의한 차나무 잔가지(부산물)의 추출물 내 지표 성분의 정량분석

Quantitative Determination of Marker Compounds in the Extracts of Camellia sinensis L. Sub-branches (Residual Products) by HPLC
키워드 :
Camellia sinensis L.,Sub-branch,Marker Compounds,HPLC,Quantitative Analysis

목차

ABSTRACT
서 언
재료 및 방법
  1. 기기 및 시약
  2. 물질의 분리 및 동정
  3. HPLC 분석 표준액 조제
  4. 검액 조제
  5. HPLC 분석조건
  6. 검량선 작성 및 검출 한계 (LOD), 정량 한계 (LQD) 측정
  7. 통계분석
결과 및 고찰
  1. 지표물질 분리 및 동정
  2. 검량선 및 검출한계, 정량한계 조사
  3. 차나무 잔가지의 추출 조건별 지표성분 함량분석
REFERENCES

초록

Background: Camellia sinensis L.(CS) is a perennial evergreen species of plant whose leaves are used to produce tea. In this plant species, the parts used are the leaves, sub-branch parts are thrown out.
Methods and Results: Ethanol extract of sub-branch parts was used for isolation of major compounds by column chromatography. Structures were identified as caffeine (1), (-)-epicatechin (2) and (-)-epicatechin gallate (3) by interpretation of spectroscopic analysis, including 1H- and 13C-NMR. High-performance liquid chromatography (HPLC) method was used to compare the quantitative level of marker compounds in various extraction solvents of sub-branch parts of CS. The content of caffeine, (-)-epicatechin, and (-)- epicatechin gallate in 30% ethanol extract showed higher value with 3.28 ± 0.57 ㎎/g, 5.53 ± 0.88 ㎎/g, and 1.29 ± 0.24 ㎎/g, respectively.
Conclusions: These results indicated that not only leaves parts but also sub-branch, could be a good source for the functional material and pharmaceutical industry.