한국약용작물학회지 Vol. 27 No. 1 (p.17-23)

일천궁과 토천궁에서 발생하는 총채벌레류의 분자동정

Molecular Identification of Thrips in Two Medicinal Crops, Cnidium officinale Makino and Ligusticum chuanxiong Hort
키워드 :
Cnidium officinale Makino,Ligusticum chuanxiong Hort,Thrips,Molecular Identification,PCR-RFLP,ITS2 Primer

목차

ABSTRACT
서 언
재료 및 방법
  1. 천궁 가해 총채벌레 피해증상 확인 및 채집
  2. Genomic DNA 추출, PCR 증폭 및 PCR-RFLP 실시
  3. BLAST 검색 및 계통도 작성
결과 및 고찰
REFERENCES

초록

Background: Cnidium officinale Makino and Ligusticum chuanxiong Hort. are important medicinal crops in Korea. However, there is insufficient information on the identification of thrips, which attack these plants. Until now, one species of thrips has been recorded as a main pest.
Methods and Results: To identify the thrips emerging in C. officinale Makino and L. chuanxiong Hort., these plants were independently cultivated in two local areas. Thirty individuals of each plant species were selected randomly and surveyed for the presence of thrips. After confirming the existence of thrips, 100 thrips individuals were collected from each crop using the beating method. To identify thrips species, we performed PCR-restriction fragment length polymorphism (RFLP)-based analysis using ITS2 primer sets. Six thrips species were identified: western flower thrips (Frankliniella occidentalis), flower thrips (F. intonsa), onion thrips (Thrips tabaci), chrysanthemum thrips (T. nigropilosus), chilli thrips (Scirtothrips dorsalis), and grass thrips (Anaphothrips obscurus). The proportion of these species differed between the host plant species.
Conclusions: Six thrips species were major pests of two medicinal crops. Integrated pest management is required to control these thrips species, and will enhance the yield and quality of C. officinale and L. chuanxiong.