한국약용작물학회지 Vol. 27 No. 1 (p.38-44)

민들레 뿌리 물 추출물의 류마티스 관절염 동물 모델에 대한 개선 효과

Water Extract of Taraxaci Radix Improves Rheumatoid Arthritis Induced by Type-II Collagen in Animal Models
키워드 :
Taraxaci radix,Autoimmune Disease,Rheumatoid Arthritis

목차

ABSTRACT
서 언
재료 및 방법
  1. 실험재료와 추출물의 제조
  2. 실험 동물 및 처치
  3. 점수화
  4. Hematoxylin과 eosin 염색
  5. Enzyme-linked immunospecific assay (ELISA)
  6. 통계처리
결과 및 고찰
  1. 제2형 콜라겐에 의해 유도된 관절의 부종에 대한 민들레뿌리 물 추출물의 개선효과
  2. 활막 염증세포 침윤과 제2형 콜라겐 자가항체에 대한민들레 뿌리 물 추출물의 개선효과
  3. 염증매개인자에 대한 민들레 뿌리 물 추출물의 개선 효과
REFERENCE

초록

Background: Taraxacum platycarpum has been used in traditional medicine in Korea to treat intoxication and edema and as a diuretic. According to previous reports, it has anti-cancer, anti-gastritis, and anti-inflammation effects. However, the improvement effect of T. platycarpum on rheumatoid arthritis has not been investigated. The anti-oxidative and anti-inflammation effects of the aerial parts of T. platycarpum are different from those of its subterranean parts. Thus, we evaluated the effect of the water extracts of Taraxaci radix (WTR) on type II collagen-induced rheumatoid arthritis (CIA) in animal models.
Methods and Results: Rheumatoid arthritis was induced by type II collagen. WTR (100㎎/㎏ and 500㎎/㎏) was administered to the animal models. Methotrexate was used as the positive control. The levels of interleukin-6, TNF-alpha, and type II collagen IgG in the animals were measured by using enzyme-linked immunosorbent assay. Treatment with 500㎎/㎏ WTR decreased the serum levels of interleukin-6, TNF-alpha, and collagen IgG in the CIA models. Moreover, treatment with WTR diminished the arthritisinduced swelling of the hind legs and monocyte infiltration in the bloodvessels of the animal models.
Conclusions: These results indicate that WTR has the potential to improve rheumatoid arthritis by reducing the levels of inflammatory cytokines such as interleukin-6 and TNF-alpha. However, further experiments are required to elucidate the influence of WTR on signal transduction in vitro and in vivo.