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축·수산·전통식품 중 황색포도상 구균의 정량적 분석을 위한 PetrifilmTM Staph Express Count Plate의 성능 평가 KCI 등재

Efficiency of PetrifilmTM Staph Express Count Plate for the Enumeration of Staphylococcus aureus in Meat, Fishery Product, and Korean Traditional Foods

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한국식품위생안전성학회지 (Journal of Food Hygiene and Safety)
한국식품위생안전성학회 (Korean Society of Food Hygiene and Safety)
초록

To enumerate Staphylococcus aureus in food, Baird-Parker Agar (BPA) is usually used in the conventional method, However it requires time and space for the preparation and plating, and incubation. Thus, use of the 3MTM PetrifilmTM Staph Express Count Plate (STX Petrifilm) might be appropriate to solve these challenging problems. The purpose of this study was to compare the efficiency of STX Petrifilm with BPA for enumeration of S. aureus in various foods. A mixture of S. aureus strains ATCC29213, ATCC25923, and ATCC13565 was inoculated on marinated pork chop, beef (chuck tender), dried filefish, semi-dried squid, rice cake, and Japchae (stir-fried glass noodles) at 2, 3, 5, and 7 Log CFU/g. S. aureus cell counts were enumerated by spread-plating on STX Petrifilm and BPA after 0 and 24 hours at 4oC (marinated pork chop, beef, semi-dried squid, and stir-fried glass noodles) and 25oC (dried filefish and rice cake). Recovery of STX Petrifilm for S. aureus from various food samples was compared with BPA, and the results showed that there were no significant differences between two selective media in all cases. The results indicated that STX Petrifilm had enough efficiency to recover S. aureus from various foods as well as saving time and space.

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ABSTRACT
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저자
  • 유윤정(숙명여자대학교 식품영양학과) | Yoonjeong Yoo (Department of Food and Nutrition, Sookmyung Women’s University)
  • 최유나(숙명여자대학교 식품영양학과) | Yuna Choi (Department of Food and Nutrition, Sookmyung Women’s University)
  • 최승호(한국쓰리엠 식품안전사업팀) | Seungho Choi (Food Safety Division, 3M Korea)
  • 방현조(한국쓰리엠 식품안전사업팀) | Hyunjo Bang (Food Safety Division, 3M Korea)
  • 윤요한(숙명여자대학교 위해분석연구센터) | Yohan Yoon (Risk Analysis Research Center, Sookmyung Women’s University)
  • 하지명(숙명여자대학교 위해분석연구센터) | Jimyeong Ha (Risk Analysis Research Center, Sookmyung Women’s University) Correspondence to
  • 이수민(숙명여자대학교 위해분석연구센터) | Soomin Lee (Risk Analysis Research Center, Sookmyung Women’s University) Correspondence to