한국식품영양학회지 Vol. 32 No. 3 (p.208-215)

택란의 일반성분, 지방산 조성 및 세포독성 효과

Proximate Analysis, Fatty Acid Composition of Lycopus lucidus Turcz. and Its Cytotoxic Effect in Cancer Cell Lines
키워드 :
Lycopus lucidus Turcz,fatty acid composition,human cancer cell,cytotoxicity

목차

Abstract
서 론
재료 및 방법
  1. 재료
  2. 추출 및 분획
  3. 일반성분 분석
  4. 지질 및 지방산 추출
  5. Gas chromatography를 이용한 지방산 분석
  6. MTT assay
  7. 통계분석
결과 및 고찰
  1. 택란 일반성분 및 지방산 조성
  2. 택란 추출물 및 분획물의 세포독성 효과
요약 및 결론
References

초록

In this paper, we investigate to determine quality characteristics, fatty acid composition and cytotoxic effect of extracts and fractions from whole Lycopus lucidus Turcz. roots. Additionally, we evaluated cytotoxic activity against the growth of human fibrosarcoma cells (HT-1080) and human gastric adenocarcinoma (AGS), human colon cancer cell (HT-29) lines using a 3-(4,5-dimethylthiazol-2-yl)-2,5-diphenyltetrazolium bromide (MTT) assay. Acetone+methylene chloride (A+M) and methanol (MeOH) extracts from L. lucidus Turcz. were obtained through solvent extraction. Then we further fractionated both extracts with n-hexane, 85% aq. MeOH, n-butanol (n-BuOH) and water. In fatty acid composition, L. lucidus Turcz. contained 33.2% of 18:1n-9 and 1.81% of 18:3n-3, respectively. The incorporation of treatment with A+M and MeOH extracts and n-hexane, 85% aq. MeOH, n-butanol (n-BuOH) and water fractions dose-dependently increased cytotoxicity against the growth of HT-1080 and AGS, HT-29 cancer cells (p<0.05). The A+M extract had a higher inhibitory effect on the growth of all cancer cells in comparison to MeOH extract. Among the fractions, the 85% aq. MeOH and n-hexane fractions showed a higher inhibitory effect after proliferating the three cancer cells. These results suggest that the 85% aq. MeOH and n-hexane fractions have a potential to inhibit the growth of human cancer cell lines.