Nelumbo nucifera Gaertn has been usedas a traditional remedy and food source in South Korea. It promotes gastrointestinal function and controls blood pressures. Nelumbo nucifera Gaertn water extracts supplement at 5, 10, 50, 100, 250, 500, 1,000 μg/mL after a 48 h pre-treatment with the mitogen (ConA or LPS) increased the mouse splenocytes proliferation. Water extract supplement also increased the cytokine production (IL-1β, TNF-α and IFN-γ), measured by a cytokine ELISA kit. For the result of in vitro study, the proliferation of splenocytes and cytokine production activated by peritoneal macrophages increased when water extracts were supplemented in the range of 50~500 μL/mL concentration. Specifically, the levels of the splenocytes proliferation, IL-1β, TNF-α and IFN-γ were the highest at 250 μL/mL concentration. This in vitro study suggestedthat supplementation with Nelumbo nucifera Gaertn water extracts may enhance the immune function by regulating the splenocyte proliferation and enhancing the cytokine production activating macrophage in vitro.

Abstract
 서 론
 재료 및 방법
  1. 재료 및 시약
  2. 마우스 비장세포의 분리 및 배양
  3. 사이토카인(IL-1β, IFN-γ, TNF-α) 분비량 측정
  4. 통계분석
 결과 및 고찰
  1. In vitro 실험에서의 연근 열수추출물이 마우스 비장세포 활성효과
  2. 연근 열수추출물이 사이토카인 분비량에 미치는 영향
 요약 및 결론
 References

• 류혜숙(상지대학교 보건과학대학 식품영양학과) | Hye-Sook Ryu (Dept. of Food and Nutrition, College of Health Sciences, Sangji University) Corresponding author