동물자원연구 제30권 3호 (p.97-104)

순종 랜드레이스의 10주령 체중 형질에 영향을 미치는 후보유전자 탐색을 위한 전장유전체 분석

Genome-wide Association Study Analysis for Explore of Candidate Genes Affecting Weight of 10 Week Old Traits in Purebred Landrace
키워드 :
Candidate gene,Genome-wide association study,Purebred landrace,Single nucleotide polymorphism marker,Weight of 10 week

목차

ABSTRACT
Ⅰ. 서론
Ⅱ. 재료 및 방법
   1. 공시 동물 및 DNA 추출
   2. 10주령 체중 형질 측정
   3. 데이터 기초분석 및 전장유전체 연관성 분석
Ⅲ. 결과 및 고찰
Ⅳ. 요약
Ⅴ. 참고문헌

초록

According to Livestock Inspection Standards, the piglets enter the feedlot at approximately 30 kg, and the inspection starts after the preliminary feeding period. The reason for applying the preliminary feeding period is to select inspection piglets with no diseases after the complete growth of the internal organs until 10 weeks of age. Furthermore, the age of 10 week is the time when the muscle fibers grow to their maximum size and the piglets are prepared for fat deposition at the later fattening period. In the study, a genome-wide association study (GWAS) was performed through the mlma command of the genome-wide complex trait analysis (GCTA) program with 703 purebred Landrace population, and the candidate genes associated with the weight of 10 week were searched. The GWAS identified 3 single nucleotide polymorphism (SNP) markers, which have a significant genome-wide suggestive level, on chromosome 6 (DIAS0002615; p-value=1.62×10-6, MARC0083933; p-value=4.94×10-6, ASGA0028717; p-value=5.40×10-6). The 2 genes (Ubiquitin protein ligase E3 component n-recognin 4; UBR4, WD and tetratricopeptide repeats 1; WDTC1) in which these 3 SNP markers are located are positional candidate genes of the weight of 10 week of the purebred Landrace population. 2 candidate genes have been reported to be associated with fattening. Therefore, the positional candidate genes in this study, UBR4 and WDTC1, are expected to be usable as genes for traits associated with the weight of 10 week weight and fattening through additional experimental research with other population.