한국식품영양학회지 Vol. 32 No. 5 (p.408-416)

LPS로 유도된 RAW264.7 세포주 염증모델에서 소리쟁이의 법제처리에 따른 항염증 효과

Anti-Inflammatory Effects of Beopje Processed Curly Dock (Rumex crispus L.) in LPS-Induced Murine RAW 264.7 Cell Lines
키워드 :
curley dock,LPS,Beopje processed,anti-inflammatory effect

목차

Abstract
서 론
재료 및 방법
   1. 실험 재료 준비
   2. 마우스 대식세포 RAW 264.7 세포주 배양
   3. 생존율 분석
   4. Nitric oxide(NO) 농도 측정
   5. Enzyme-Linked Immunosorbent Assay(ELISA)
   6. Real Time - Quantitative Polymerase Chain Reaction(RT-qPCR)
   7. 통계처리
결과 및 고찰
   1. 세포 생존율에 미치는 영향
   2. Raw 264.7 세포에서 NO 생성
   3. Raw 264.7 세포에서pro-inflammatory cytokine의 함량
   4. Raw 264.7 세포에서 iNOS, COX-2의 mRNA 발현
요약 및 결론
References

초록

This study investigated the anti-inflammatory effects of processed (Beopje) curly dock (Rumex crispus L.) in LPS (lipopolysaccharide)-stimulated murine RAW 264.7 cells. The experimental group was classified into five groups : LPS no treatment, CD (curly dock), CD-B (CD processed through Beopje), LPS, LPS+CD-B (LPS+CD processed through Beopje) and LPS+CD (LPS+CD). Treatment of the Raw 264.7 cell lines using LPS led to a significant increase in NO production, pro-inflammatory cytokines (TNF-α, IL-6 and IL-1β), and inflammation related genes (COX-2 and iNOS). Investigation of the inhibitory effects of CD and processed CD on NO production and expression of iNOS and COX-2 was done in LPS-induced RAW 264.7 cells. There was significant inhibition of NO production by LPS+CD and LPS+CD-B in a dose-dependent manner (p<0.05). Particularly, LPS+CD-B exhibited reduced mRNA expression of iNOS and COX-2 and NO production as compared to LPS+CD in Raw 264.7 cell lines (p<0.05). These results may explain some known biological activities of curly dock including the anti-inflammatory effects. CD-B in particular exhibited the highest anti-inflammatory effects of inhibiting production of NO, through the regulation of inflammatory related genes and pro-inflammatory cytokines. These results of Beopje processing might help decrease the anti-biological effects and increase several active substances of curly dock