한국식품영양학회지 Vol. 32 No. 5 (p.553-559)

도라지 추출성분의 항균․항산화 및 탈모예방 효과

Evaluation of Antibacterial, Antioxidant Fractionalities and Hair Loss Prevention Effect of Platycodon grandiflorum
키워드 :
Platycodon grandiflorum,NO,PGE2,DPPH,DHT

목차

Abstract
서 론
재료 및 방법
   1. 원료 및 추출공정
   2. DPPH radical 소거능
   3. ABTS radical 소거능
   4. 세포 배양
   5. Nitric oxide 생성량 측정
   6. PGE2 생성량 측정
   7. 세포 배양
   8. 세포재생 효능 평가
   9. 균주 배양
   10. 항균활성 측정
   11. Dihydrotestosterone(DHT) 저해능 평가
   12. 통계분석
결과 및 고찰
   1. 도라지 추출성분의 항산화능
   2. 도라지 추출성분의 항염증 효과
   3. 도라지 추출성분의 피부세포 재생 효과
   4. 도라지 추출성분의 항균 효과
   5. 도라지 추출성분의 DHT 생성 저해 효과
요약 및 결론
References

초록

To investigate the effects of antioxidant activities and hair loss prevention of extracts from Platycodon grandiflorum, we’ve prepared chloroform (CF) and ethylacetate fractions (EA) extracted from P. grandiflorum. In the results of DPPH radical scavenging assay, the two fractions showed dose-dependent antioxidant activities. Furthermore, in the ABTS assay, the two fractions exhibited the inhibitory effect over 90% at 10, 50, 100, 200 mg/mL. To investigate the inflammation inhibitory effect, we used RAW264.7 cells, these extracts were inhibited inflammatory reaction by suppressing the production of nitric oxide (NO) and prostaglandin E2 (PGE2) in dose-dependent manner. In the assay of HaCaT cells’ proliferation, the 24 hr treatment of the extracts significantly accelerarted cell proliferation in the range of concentrations used. The two fractions inhibited the proliferation of Malssezia furfur, the bacteria induce the dandruff. Finally, the CF could significantly inhibit the DHT production at 1, 10, 50, 100 μg/mL, but EA showed the inhibitory effect at the concentration over 50 μg/mL. The overall results of this study suggest that the chloroform (CF) and ethylacetate fractions (EA) from P. grandiflorum could be a useful raw material for the hair loss prevention products.