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렌즈콩(Lens culinaris) 추출물이 HepG2 인간 간암 세포에서 Proteasome 활성과 Nuclear Factor κB 활성화에 미치는 영향 KCI 등재

Effects of Lentils(Lens culinaris) Extract on Proteasome Activity and Nuclear Factor κB Activation in HepG2 Human Liver Cancer Cells

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한국식품영양학회지 (The Korean Journal of Food And Nutrition)
한국식품영양학회 (The Korean Society of Food and Nutrition)
초록

Proteasome inhibitors can improve the efficiency of cancer treatments by inhibiting nuclear factor κB(NF-κB) activation in cancer cells. Lentils are a type of beans of which consumption of such beans is increasing. The purpose of this study was to investigate the effects of lentils extract (LE) on the proteasomal activities, NF-κB activation, and cell cycle in HepG2 human liver cancer cells. LE treatments inhibited proteasomal activities at concentrations of 10, 50, and 100 μg/mL respectively, and repressed NF-κB activation at concentrations of 1, 10, and 100 μg/mL respectively, in HepG2 cells. LE treatments at concentrations of 1, 10, and 100 μg/mL respectively, increased sub-G1 cell population in HepG2 cells, which may be the result of apoptosis. The results suggest that LE inhibited NF-κB activation partially with its proteasome inhibitory activities, and the increase of sub-G1 cell population was induced partially, by inhibition of NF-κB activation in HepG2 cells.

목차
Abstract
서 론
재료 및 방법
    1. 재료
    2. 렌즈콩 추출물 제조
    3. 세포 배양
    4. 세포 독성 측정
    5. Proteasome 억제 활성 측정
    6. NF-κB 활성화 측정
    7. Cell cycle 분석
    8. 통계처리
결과 및 고찰
    1. 렌즈콩 추출물의 세포 독성
    2. 렌즈콩 추출물의 proteasome 억제 활성
    3. 렌즈콩 추출물이 NF-κB 활성화에 미치는 영향
    4. 렌즈콩추출물이HepG2 세포의cell cycle에미치는영향
요약 및 결론
References
저자
  • 민수영(성신여자대학교 식품영양학과) | Sooyeong Min (Dept. of Food and Nutrition, Sungshin Women’s University)
  • 윤현근(성신여자대학교 바이오식품공학과) | Hyungeun Yoon (Dept. of Food Science and Biotechnology, Sungshin Women’s University) Corresponding author