한국식품영양학회지 Vol. 32 No. 5 (p.565-570)

렌즈콩(Lens culinaris) 추출물이 HepG2 인간 간암 세포에서 Proteasome 활성과 Nuclear Factor κB 활성화에 미치는 영향

Effects of Lentils(Lens culinaris) Extract on Proteasome Activity and Nuclear Factor κB Activation in HepG2 Human Liver Cancer Cells
키워드 :
proteasome inhibitor,nuclear factor κB,lentils extract,HepG2 cells

목차

Abstract
서 론
재료 및 방법
   1. 재료
   2. 렌즈콩 추출물 제조
   3. 세포 배양
   4. 세포 독성 측정
   5. Proteasome 억제 활성 측정
   6. NF-κB 활성화 측정
   7. Cell cycle 분석
   8. 통계처리
결과 및 고찰
   1. 렌즈콩 추출물의 세포 독성
   2. 렌즈콩 추출물의 proteasome 억제 활성
   3. 렌즈콩 추출물이 NF-κB 활성화에 미치는 영향
   4. 렌즈콩추출물이HepG2 세포의cell cycle에미치는영향
요약 및 결론
References

초록

Proteasome inhibitors can improve the efficiency of cancer treatments by inhibiting nuclear factor κB(NF-κB) activation in cancer cells. Lentils are a type of beans of which consumption of such beans is increasing. The purpose of this study was to investigate the effects of lentils extract (LE) on the proteasomal activities, NF-κB activation, and cell cycle in HepG2 human liver cancer cells. LE treatments inhibited proteasomal activities at concentrations of 10, 50, and 100 μg/mL respectively, and repressed NF-κB activation at concentrations of 1, 10, and 100 μg/mL respectively, in HepG2 cells. LE treatments at concentrations of 1, 10, and 100 μg/mL respectively, increased sub-G1 cell population in HepG2 cells, which may be the result of apoptosis. The results suggest that LE inhibited NF-κB activation partially with its proteasome inhibitory activities, and the increase of sub-G1 cell population was induced partially, by inhibition of NF-κB activation in HepG2 cells.