대한구강악안면병리학회지 제43권 제5호 (p.171-178)

|원 저|
생약 복합 추출물의 인간 골수 유래 중간엽 줄기세포에서의 골 분화에 미치는 영향

The Effect of Natural-Herb Mixture Extract on Osteogenic Differentiation in Human Mesenchymal Stem cells
키워드 :
NME extract,Human bone-marrow mesenchymal stem cells,Osteogenic differentiation,APA

목차

<Abstract>
Ⅰ. INTRODUCTION
Ⅱ. MATERIALS and METHODS
   1. 실험재료
   2. 세포배양
   3. 세포의 분화
   4. 세포 생존률 측정
   5. Alkaline phosphatase (ALP) 염색
   6. Alizarin Red S 염색법
   7. RNA isolation와 cDNA 합성
   8. RT-PCR
   9. 통계 처리
Ⅲ. RESULTS
   1. NME 농도에 따른 인간 골수 유래 중간엽 줄기세포 증식 정도 확인
   2. NME 처리에 의한 ALP 활성 확인
   3. NME 처리에 의한 미네랄 형성정도 확인
   4. NME 처리에 의한 인간 골수 유래 중간엽 줄기세포의 골 분화 마커의 발현
Ⅳ. DISCUSSION
REFERENCES

초록

Herbal medicine has been the basis for medical treatments through much of human history, and such traditional medicine is still widely practiced today. Modern medicine makes use of many plant-derived compounds as the basis for pharmaceutical drugs. In traditionally, Achyranthes aspera, Safflower (Carthamus tinctorius) seed and Acanthopanax senticosus have been used for the treatment and prevention of bone-related diseases. In this study, we investigated the pharmacological effect of mixture of Achyranthes aspera, Safflower (Carthamus tinctorius) seed and Acanthopanax senticosus and the other herbs. Two types of enzymes were used to enhance the extraction components of amino acid, mineral content, free sugar, and flavor recovery in extracting natural herbal mixtures(NME). We evaluated regulation of osteogenic differentiation in human bone marrow mesenchymal stem cells using alkaline phosphatase staining, alizarin red S staining and RT-PCR. The CCK-8 assay indicated that NME had no cytotoxicity but increased cell survival. In addition, NME promoted the mineralization and expression of osteogenic differention marker genes in human bone marrow mesenchymal stem cells. Therefore, NME has an effect of promoting proliferation and osteogenic differentiation of human mesenchymal stem cell.